• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA.
  • Amino Acid Range: Ala24-Asn239
Catalog #TRL-HM2R1
Price
Regular price $23980
Regular price Sale price $23980
/
Size
Avantor VWR Fisher Scientific

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Human TRAIL R1/DR4/TNFRSF10A Protein (TRL-HM2R1) Summary

Product Description

 Recombinant Human TRAIL R1/DR4/TNFRSF10A Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Ala24-Asn239.

Product Background

 Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induces apoptosis selectively via its interaction with the death receptors TRAILR1/DR4 and TRAILR2/DR5 in a wide range of cancers, while sparing normal cells. Despite its tremendous potential for cancer therapeutics, the translation of TRAIL into the clinic has been confounded by TRAIL-resistant cancer populations.

Human TRAIL R1/DR4/TNFRSF10A Protein (TRL-HM2R1) Specifications/Details

Protein
 TRAIL R1/DR4/TNFRSF10A
Synonyms
 DR4; CD261; TNFRSF10A; TRAIL-R; APO2; TRAIL R1; MGC9365
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Ala24-Asn239
Molecular Weight
 The protein has a predicted MW of 48.8 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA. Immobilized Human TRAIL, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TRAIL R1, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA.
Human TRAIL R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human TRAIL R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TRAIL R1 is greater than 95% as determined by SEC-HPLC. The purity of Human TRAIL R1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...