Biotinylated Human CD200 R1/CRTR2 Protein (CD2-HM4R1B)
Biotinylated Human CD200 R1/CRTR2 Protein (CD2-HM4R1B)
Biotinylated Human CD200 R1/CRTR2 Protein (CD2-HM4R1B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human CD200 R1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD200, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ala27-Leu266
Catalog #CD2-HM4R1B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human CD200 R1/CRTR2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Leu266.

Product Background

CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

CD200 R1/CRTR2

Synonyms

CD200R; CRTR2; MOX2R; OX2R; CD200 R1; CD200 receptor 1; HCRTR2; MOX2 receptor

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Ala27-Leu266

Molecular Weight

The protein has a predicted MW of 29.7 kDa. Due to glycosylation, the protein migrates to 52-70 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Biotinylated Human CD200 R1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD200, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human CD200 R1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD200, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human CD200 R1, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD200, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA (QC Test).
Biotinylated Human CD200 R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CD200 R1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD200 R1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human CD200 R1 is greater than 95% as determined by SEC-HPLC.

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