- Express System: HEK293
- Product Tag: N-His-Avi,N-Flag
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Biotinylated Human CD40 Ligand Trimer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 56.2ng/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Met113-Leu261
Product Description |
Recombinant Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with His tag, Avi tag and Flag tag at the N-terminus.It contains Met113-Leu261. |
Product Background |
CD40 ligand or CD40L, also called CD154,- is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells. |
Product Category |
Recombinant Protein |
Target |
CD40 Ligand/TNFSF5 Trimer |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Met113-Leu261 |
Molecular Weight |
The protein has a predicted MW of 54.51 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human CD40 Ligand Trimer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 56.2ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Avi,N-Flag |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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