Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein (CDL-HM440B)
  • Express System: HEK293
  • Product Tag: N-His-Avi,N-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human CD40 Ligand Trimer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 56.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Met113-Leu261
Catalog #CDL-HM440B
Regular price $525.00
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Product Description
Recombinant Biotinylated Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with His tag, Avi tag and Flag tag at the N-terminus.It contains Met113-Leu261.
Product Background
CD40 ligand or CD40L, also called CD154,- is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
Product Category
Recombinant Protein
Target
CD40 Ligand/TNFSF5 Trimer
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Met113-Leu261
Molecular Weight
The protein has a predicted MW of 54.51 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human CD40 Ligand Trimer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 56.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
N-His-Avi,N-Flag
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human CD40 Ligand Trimer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 56.2ng/ml determined by ELISA.
Biotinylated Human CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.

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