Name: Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein (CDL-HM440B)
Brand: KactusBio
SKU: CDL-HM440B-100UG
Price: 577.5 USD
Availability: InStock

ELISA binding curve for biotinylated human CD40 ligand (trimer), His Tag, showing mean absorbance (OD450) versus log concentration of human CD40, hFc tag.

Immobilized Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 56.2ng/ml determined by ELISA.

Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: N-His-Avi,N-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 56.2ng/ml determined by ELISA.
Amino Acid Range: Met113-Leu261

Catalog #CDL-HM440B

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein (CDL-HM440B) Summary

Product Description	Recombinant Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with His tag, Avi tag and Flag tag at the N-terminus.It contains Met113-Leu261 trimer design.
Product Background	CD40 ligand or CD40L, also called CD154,- is a protein that is primarily expressed on activated T cells and is a member of the TNF superfamily of molecules. It binds to CD40 (protein) on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. In total CD40L has three binding partners: CD40, α5β1 integrin and αIIbβ3. CD154 acts as a costimulatory molecule and is particularly important on a subset of T cells called T follicular helper cells.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand/TNFSF5 Trimer Protein (CDL-HM440B) Specifications/Details

Protein	CD40 Ligand/TNFSF5
Synonyms	CD40L; CD40LG; CD154; TNFSF5; TNFSF5IMD3; CD40LIGM; gp39; HIGM1; T-BAM; TRAP; IGM; IMD3; CD40 Ligand
Accession	P29965(Human) / G7Q1T4(Cynomolgus) / P63304(Rhesus macaque)
Species	Human/Cynomolgus/Rhesus macaque
Biotinylated	yes
Amino Acid Range	Met113-Leu261
Molecular Weight	The protein has a predicted MW of 54.51 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 56.2ng/ml determined by ELISA.
Product Tag	N-His-Avi,N-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD40, hFc Tag with the EC50 of 56.2ng/ml determined by ELISA.
	Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Human/Cynomolgus/Rhesus macaque CD40 Ligand Trimer is greater than 95% as determined by SEC-HPLC.