Biotinylated Human GPRC5D Protein-Nanodisc (GPR-HM45PB)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Biotinylated: yes
  • Activity: Immobilized Anti-GPRC5D Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GPRC5D Nanodisc, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). The affinity constant of 6.30 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Met1-Val345

Catalog: GPR-HM45PB

$1,875.00
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Product Description Recombinant Biotinylated Human GPRC5D Protein-Nanodisc is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Met1-Val345.

Product Background

Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein–coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA.

Product Category

Recombinant Protein

Target

GPRC5D

Gene/Protein ID

Q9NZD1-1

Species

Human

Exact Sequence

Met1-Val345
Molecular Weight The protein has a predicted MW of 42.10 kDa.

Activity

Immobilized Anti-GPRC5D Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GPRC5D Nanodisc, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). The affinity constant of 6.30 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22 um filtered solution in PBS (pH 7.4).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Biotinylated Human GPRC5D Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-GPRC5D Antibody with an affinity constant of 6.30 nM as determined in SPR assay (Biacore T200).
Immobilized Anti-GPRC5D Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human GPRC5D Nanodisc, His Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).
Immobilized Human CD3E&CD3D, His Tag at 2ug/ml (100ul/well) on the plate, add serial dilutions of Anti-Human CD3×GPRC5D Bispecific Antibody, hFc Tag and then add Biotinylated Human GPRC5D Nanodisc, His Tag at 5ug/ml. Detection was performed using HRP-conjugated streptavidin with the EC50 of 0.28ug/ml determined by ELISA.
Loaded Biotinylated Human GPRC5D Nanodisc, His Tag on SA-Biosensor, can bind Anti-GPRC5D Antibody with an affinity constant of 1.16 nM as determined in BLI assay (Gator Prime).

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