Product Description |
Recombinant Biotinylated Human GPRC5D Protein-Nanodisc is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Met1-Val345. |
Product Background |
Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein–coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA. |
Product Category |
Recombinant Protein |
Target |
GPRC5D |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Met1-Val345 |
Molecular Weight |
The protein has a predicted MW of 42.10 kDa. |
Activity |
Immobilized Biotinylated Human GPRC5D Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA (QC Test). The affinity constant of 6.30 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Liquid |
Shipping |
Shipped with dry ice. |
Formulation |
Supplied as 0.22 um filtered solution in PBS (pH 7.4). |
Stability And Storage |
Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Biotinylated Human GPRC5D Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-GPRC5D Antibody with an affinity constant of 6.30 nM as determined in SPR assay (Biacore T200). |
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Immobilized Biotinylated Human GPRC5D Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA (QC Test). |
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Immobilized Human CD3E&CD3D, His Tag at 2ug/ml (100ul/well) on the plate, add serial dilutions of Anti-Human CD3×GPRC5D Bispecific Antibody, hFc Tag and then add Biotinylated Human GPRC5D Nanodisc, His Tag at 5ug/ml. Detection was performed using HRP-conjugated streptavidin with the EC50 of 0.28ug/ml determined by ELISA. |
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Loaded Biotinylated Human GPRC5D Nanodisc, His Tag on SA-Biosensor, can bind Anti-GPRC5D Antibody with an affinity constant of 1.16 nM as determined in BLI assay (Gator Prime). |
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