Name: Biotinylated Human GPRC5D Protein-Nanodisc (GPR-HM45PB)
Brand: KactusBio
SKU: GPR-HM45PB-100UG
Price: 1875.0 USD
Availability: InStock

Biotinylated Human GPRC5D Protein-Nanodisc (GPR-HM45PB)

Biotinylated Human GPRC5D Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-GPRC5D Antibody with an affinity constant of 6.30 nM as determined in SPR assay (Biacore T200).

Immobilized Biotinylated Human GPRC5D Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA (QC Test).

Immobilized Human CD3E&CD3D, His Tag at 2ug/ml (100ul/well) on the plate, add serial dilutions of Anti-Human CD3×GPRC5D Bispecific Antibody, hFc Tag and then add Biotinylated Human GPRC5D Nanodisc, His Tag at 5ug/ml. Detection was performed using HRP-conjugated streptavidin with the EC50 of 0.28ug/ml determined by ELISA.

Loaded Biotinylated Human GPRC5D Nanodisc, His Tag on SA-Biosensor, can bind Anti-GPRC5D Antibody with an affinity constant of 1.16 nM as determined in BLI assay (Gator Prime).

Express System: HEK293
Product Tag: C-His-Avi
Activity: Immobilized Biotinylated Human GPRC5D Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA (QC Test). The affinity constant of 6.30 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Met1-Val345

Catalog #GPR-HM45PB

Size

100UG

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Product Description	Recombinant Biotinylated Human GPRC5D Protein-Nanodisc is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Met1-Val345.
Product Background	Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein–coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA.
Product Category	Recombinant Protein / Antibody discovery / Multiple transmembrane proteins / Nanodisc

Protein	GPRC5D
Synonyms	GPRC5D; MGC129713; MGC129714; orphan G-protein coupled receptor
Accession	Q9NZD1-1
Species	Human
Biotinylated	yes
Amino Acid Range	Met1-Val345
Molecular Weight	The protein has a predicted MW of 42.10 kDa.
Activity	Immobilized Biotinylated Human GPRC5D Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA (QC Test). The affinity constant of 6.30 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22 um filtered solution in PBS (pH 7.4). Notice: Not recommended for immunization and flow cytometry in mammalian cells.
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Biotinylated Human GPRC5D Nanodisc, His Tag captured on CM5 Chip via Streptavidin can bind Anti-GPRC5D Antibody with an affinity constant of 6.30 nM as determined in SPR assay (Biacore T200).
	Immobilized Biotinylated Human GPRC5D Nanodisc, His Tag at 5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-GPRC5D Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA (QC Test).
	Immobilized Human CD3E&CD3D, His Tag at 2ug/ml (100ul/well) on the plate, add serial dilutions of Anti-Human CD3×GPRC5D Bispecific Antibody, hFc Tag and then add Biotinylated Human GPRC5D Nanodisc, His Tag at 5ug/ml. Detection was performed using HRP-conjugated streptavidin with the EC50 of 0.28ug/ml determined by ELISA.
	Loaded Biotinylated Human GPRC5D Nanodisc, His Tag on SA-Biosensor, can bind Anti-GPRC5D Antibody with an affinity constant of 1.16 nM as determined in BLI assay (Gator Prime).