Biotinylated Human IL-13Ra1 Protein (ILR-HM4R1B)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Biotinylated Human IL-13Ra1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Ala27-Thr343

Catalog: ILR-HM4R1B

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Product Description Recombinant Biotinylated Human IL-13Ra1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala27-Thr343.

Product Background

Two type 1 membrane proteins belonging to the hemopoietin receptor family have been cloned and shown to bind IL-13 with differing affinities. The lower affinity IL-13 binding protein, previously designated IL-13 R alpha, IL-13 R alpha ' or NR4, is now referred to as IL-13 R alpha 1. The high-affinity IL-13 binding protein, previously also designated IL-13 R or IL-13 R alpha ', is now referred to as IL-13 R alpha 2.

Product Category

Recombinant Protein

Target

IL-13Ra1
Synonyms IL-13 R alpha 1; IL13R; IL13RA; CT19; IL13RA1; NR4; CD213A1; AI882074

Gene/Protein ID

P78552-1

Species

Human

Exact Sequence

Ala27-Thr343
Molecular Weight The protein has a predicted MW of 39.5 kDa. Due to glycosylation, the protein migrates to 55-75 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Biotinylated Human IL-13Ra1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human IL-13Ra1, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human IL-13, hFc Tag with the EC50 of 15.8ng/ml determined by ELISA.
The purity of-Biotinylated IL-13Ra1 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human IL-13Ra1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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