Product Description |
Recombinant Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Phe31-Val992(ITGAV) acidic tail & Glu43-Arg684(ITGB8) basic tail. |
Product Background |
Deletions of the genes encoding the integrin αVβ8 (Itgav, Itgb8) have been shown to result in abnormal vascular development in the CNS, including prenatal and perinatal hemorrhage. Other work has indicated that a major function of this integrin in vivo is to promote TGFβ activation. |
Product Category |
Recombinant Protein |
Target |
ITGAV&ITGB8 |
Synonyms |
αVβ8; Integrin alpha V beta 8; TGAV&ITGB8;Integrin alpha V β 8 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Phe31-Val992(ITGAV) acidic tail & Glu43-Arg684(ITGB8) basic tail |
Molecular Weight |
The protein has a predicted MW of 114 kDa (ITGAV)&76 kDa (ITGB8) kDa. Due to glycosylation, the protein migrates to 85-145 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Latent TGF beta 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB8, His Tag with the EC50 of 9.62ug/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human Latent TGF beta 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB8, His Tag with the EC50 of 9.62ug/ml determined by ELISA (QC Test). |
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Immobilized Biotinylated Human ITGAV&ITGB8, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. |
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Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer is greater than 95% as determined by SEC-HPLC. |
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