Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein (ITG-HM4V8B)
Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein (ITG-HM4V8B)
Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein (ITG-HM4V8B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human ITGAV&ITGB8, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Phe31-Val992(ITGAV) acidic tail & Glu43-Arg684(ITGB8) basic tail
Catalog #ITG-HM4V8B
Regular price $660.00
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Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein (ITG-HM4V8B) Summary

Product Description

Recombinant Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus of ITGAV.It contains Phe31-Val992(ITGAV) acidic tail and Glu43-Arg684(ITGB8) basic tail.

Product Background

Deletions of the genes encoding the integrin αVβ8 (Itgav, Itgb8) have been shown to result in abnormal vascular development in the CNS, including prenatal and perinatal hemorrhage. Other work has indicated that a major function of this integrin in vivo is to promote TGFβ activation.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein (ITG-HM4V8B) Specifications/Details

Protein
Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer
Synonyms
αVβ8; Integrin alpha V beta 8; TGAV&ITGB8;Integrin alpha V β 8
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Phe31-Val992(ITGAV) acidic tail & Glu43-Arg684(ITGB8) basic tail
Molecular Weight
The protein has a predicted MW of 114 kDa (ITGAV) and 76 kDa (ITGB8). Due to glycosylation, the protein migrates to 85-145 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human ITGAV&ITGB8, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human ITGAV&ITGB8, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA. Immobilized Biotinylated Human ITGAV&ITGB8, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-alpha V Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA.
Immobilized Human Latent TGF beta 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB8, His Tag with the EC50 of 9.62ug/ml determined by ELISA. Immobilized Human Latent TGF beta 1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB8, His Tag with the EC50 of 9.62ug/ml determined by ELISA.
Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer is greater than 95% as determined by SEC-HPLC.
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