• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human CD58, His Avi Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD2, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA.
  • Amino Acid Range: Phe29-Arg215
Catalog #CD5-HM418B-UL
Price
Regular price $635.80
Regular price Sale price $635.80
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human CD58 Protein, Ultra Low Endotoxin (CD5-HM418B-UL) Summary

Product Description

 Recombinant Biotinylated Human CD58 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Phe29-Arg215.

Product Background

 Loss of CD58 is a common mechanism for tumor immune evasion in lymphoid malignancies. CD58 loss is known to occur due to both genetic and non-genetic causes; therefore, we hypothesized that restoring CD58 expression in lymphoma cells may be an effective treatment approach. EZH2 is involved in the epigenetic silencing of CD58 in lymphoma cells as a mechanism for tumor immune escape, and EZH2 inhibitors are able to restore epigenetically suppressed CD58 expression.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Biotinylated Human CD58 Protein, Ultra Low Endotoxin (CD5-HM418B-UL) Specifications/Details

Protein
 CD58
Synonyms
 Lymphocyte function-associated antigen 3;Ag3;CD58;LFA3
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Phe29-Arg215
Molecular Weight
 The protein has a predicted MW of 24.37 kDa. Due to glycosylation, the protein migrates to 35-58 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human CD58, His Avi Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD2, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA.
Product Tag
 C-His-Avi

Fluorophore

 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 0.01 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human CD58, His Avi Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD2, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA. Immobilized Biotinylated Human CD58, His Avi Tag at 1ug/ml(100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Human CD2, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA.
Biotinylated Human CD58 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CD58 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Biotinylated Human CD58, His-Avi Tag on Steptavidin-Biosensor can bind Human CD2, hFc Tag (Cat. CD2-HM202) with an affinity constant of 6.46 nM as determined in BLI assay. Loaded Biotinylated Human CD58, His-Avi Tag on Steptavidin-Biosensor can bind Human CD2, hFc Tag (Cat. CD2-HM202) with an affinity constant of 6.46 nM as determined in BLI assay.
The purity of Biotinylated Human CD58 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human CD58 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...