Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein (MHC-HM418B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody, hFc Tag with the EC50 of 0.29ug/ml determined by ELISA (QC Test). The affinity constant of 0.14 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and VVVGAVGVGK peptide
Catalog #MHC-HM418B
Regular price $310.00
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Product Description
Recombinant Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M) and VVVGAVGVGK peptide.
Product Background
Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.
Product Category
Recombinant Protein
Target
HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer
MHC Form
Monomer
Allele
HLA-A*03:01
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and VVVGAVGVGK peptide
Molecular Weight
The protein has a predicted MW of 50.2 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody, hFc Tag with the EC50 of 0.29ug/ml determined by ELISA (QC Test). The affinity constant of 0.14 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer, His Tag captured on CM5 Chip via anti-his antibody can bind Anti-HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody with an affinity constant of 0.14 uM as determined in SPR assay (Biacore T200).
Immobilized Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody, hFc Tag with the EC50 of 0.29ug/ml determined by ELISA (QC Test).
Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Monomer is greater than 95% as determined by SEC-HPLC.

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