Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein (SEM-HM44DB)
Catalog: SEM-HM44DB
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Product Description | Recombinant Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met22-Arg734. |
Product Background |
Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression. |
Product Category |
Recombinant Protein |
Target |
Semaphorin 4D/SEMA4D/CD100 |
Synonyms | Semaphorin-4D; CD100; SEMA4D; C9orf164; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141; SEMAJ; coll-4 |
Accession | |
Species |
Human |
Amino Acid Range |
Met22-Arg734 |
Molecular Weight | The protein has a predicted MW of 82.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Biotinylated Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 6.9ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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