• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 6.9ng/ml determined by ELISA.
  • Amino Acid Range: Met22-Arg734
Catalog #SEM-HM44DB
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein (SEM-HM44DB) Summary

Product Description

 Recombinant Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met22-Arg734.

Product Background

 Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression.

Biotinylated Human Semaphorin 4D/SEMA4D/CD100 Protein (SEM-HM44DB) Specifications/Details

Protein
 Semaphorin 4D/SEMA4D/CD100
Synonyms
 Semaphorin-4D; CD100; SEMA4D; C9orf164; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141; SEMAJ; coll-4
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Met22-Arg734
Molecular Weight
 The protein has a predicted MW of 82.1 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 6.9ng/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Biotinylated Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 6.9ng/ml determined by ELISA. Immobilized Biotinylated Human Semaphorin 4D, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 6.9ng/ml determined by ELISA.
Biotinylated Human Semaphorin 4D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human Semaphorin 4D on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human Semaphorin 4D is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human Semaphorin 4D is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...