Product Description |
Recombinant Biotinylated Human Siglec-2/CD22 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp20-Arg687. |
Product Background |
CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. CD22 a member of the immunoglobulin superfamily. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice. |
Product Category |
Recombinant Protein / Antibody discovery / CAR-T targets |
Protein |
Siglec-2/CD22 |
Synonyms |
CD22 molecule; CD22; Siglec-2; B-cell receptor CD22; BL-CAM; SIGLEC2; SIGLEC2FLJ22814 |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Asp20-Arg687 |
Molecular Weight |
The protein has a predicted MW of 78.1 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Biotinylated Human Siglec-2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Biotinylated Human Siglec-2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test). |
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Biotinylated Human Siglec-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.. |
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The purity of Biotinylated Human Siglec-2 is greater than 95% as determined by SEC-HPLC. |
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