Biotinylated Human Siglec-2/CD22 Protein (SIG-HM412B)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Anti-Siglec-2 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-2, His Tag with the EC50 of 19.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp20-Arg687

Catalog: SIG-HM412B

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Product Description Recombinant Biotinylated Human Siglec-2/CD22 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Asp20-Arg687.
Product Category Recombinant Protein
Target Siglec-2/CD22
Synonyms CD22 molecule; CD22; Siglec-2; B-cell receptor CD22; BL-CAM; SIGLEC2; SIGLEC2FLJ22814
Gene/Protein ID P20273-1
Species Human
Exact Sequence Asp20-Arg687
Molecular Weight The protein has a predicted MW of 78.1 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.
Activity Immobilized Anti-Siglec-2 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-2, His Tag with the EC50 of 19.1ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Anti-Siglec-2 Antibody, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Siglec-2, His Tag with the EC50 of 19.1ng/ml determined by ELISA.
The purity of Biotinylated Human Siglec-2 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human Siglec-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%..

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