Name: Biotinylated Human Siglec-2/CD22 Protein (SIG-HM412B)
Brand: KactusBio
SKU: SIG-HM412B-100UG
Price: 577.5 USD
Availability: InStock

Biotinylated Human Siglec-2/CD22 Protein (SIG-HM412B)

Immobilized Biotinylated Human Siglec-2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test).

Biotinylated Human Siglec-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..

The purity of Biotinylated Human Siglec-2 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Biotinylated Human Siglec-2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Asp20-Arg687

Catalog #SIG-HM412B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Human Siglec-2/CD22 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp20-Arg687.
Product Background	CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. CD22 a member of the immunoglobulin superfamily. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	Siglec-2/CD22
Synonyms	CD22 molecule; CD22; Siglec-2; B-cell receptor CD22; BL-CAM; SIGLEC2; SIGLEC2FLJ22814
Accession	P20273-1
Species	Human
Biotinylated	yes
Amino Acid Range	Asp20-Arg687
Molecular Weight	The protein has a predicted MW of 78.1 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Biotinylated Human Siglec-2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Biotinylated Human Siglec-2, His Tag at 1ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 9.2ng/ml determined by ELISA (QC Test).
	Biotinylated Human Siglec-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..
	The purity of Biotinylated Human Siglec-2 is greater than 95% as determined by SEC-HPLC.