Biotinylated Human TNFSF15 Protein (Primary Amine Labeling) (FSF-HM115B)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human TNFSF15, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu72-Leu251
Catalog #FSF-HM115B
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Product Description
 Recombinant Biotinylated Human TNFSF15 Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the N-terminus.It contains Leu72-Leu251.
Product Background
 TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
Product Category
 Recombinant Protein
Target
 TNFSF15
Synonyms
 TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Leu72-Leu251
Molecular Weight
 The protein has a predicted MW of 21.57 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Biotinylated Human TNFSF15, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
 N-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Biotinylated Human TNFSF15, His Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 5.2ng/ml determined by ELISA.
Immobilized Mouse DR3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human TNFSF15, His Tag with the EC50 of 0.69ug/ml determined by ELISA (QC Test).
Biotinylated Human TNFSF15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human TNFSF15 is greater than 95% as determined by SEC-HPLC.

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