- Express System: HEK293
- Product Tag: C-His-Avi
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: Immobilized Human NKG2D, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ULBP-1, His Tag with the EC50 of 0.14ug/ml determined by ELISA. See testing image for detail.
- Amino Acid Range: Gly26-Pro215
Product Description |
Recombinant Biotinylated Human ULBP-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly26-Pro215. |
Product Background |
Cell surface proteins major histocompatibility complex (MHC) class I-related chain A (MICA) and UL16-binding proteins (ULBP) 1, 2, and 3 are up-regulated upon infection or tumor transformation and can activate human natural killer (NK) cells. |
Product Category |
Recombinant Protein |
Target |
ULBP-1 |
Synonyms |
ALCAN-beta; NKG2D ligand 1; N2DL-1; NKG2DL1; MULT-1; NKG2DL; AET1I; ULBP1; ULBP-1;ALCAN-β |
Accession |
|
Species |
Human |
Biotinylated |
yes |
Amino Acid Range |
Gly26-Pro215 |
Molecular Weight |
The protein has a predicted MW of 25.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human NKG2D, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ULBP-1, His Tag with the EC50 of 0.14ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20mM PB, 0.5M NaCl, 0.1M L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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