• Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NKG2D, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ULBP-1, His Tag with the EC50 of 0.14ug/ml determined by ELISA.
  • Amino Acid Range: Gly26-Pro215
Catalog #ULB-HM4P1B
Price
Regular price $57750
Regular price Sale price $57750
/
Size
Avantor VWR Fisher Scientific

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Biotinylated Human ULBP-1 Protein (ULB-HM4P1B) Summary

Product Description

 Recombinant Biotinylated Human ULBP-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly26-Pro215.

Product Background

 Cell surface proteins major histocompatibility complex (MHC) class I-related chain A (MICA) and UL16-binding proteins (ULBP) 1, 2, and 3 are up-regulated upon infection or tumor transformation and can activate human natural killer (NK) cells.

Biotinylated Human ULBP-1 Protein (ULB-HM4P1B) Specifications/Details

Protein
 ULBP-1
Synonyms
 ALCAN-beta; NKG2D ligand 1; N2DL-1; NKG2DL1; MULT-1; NKG2DL; AET1I; ULBP1; ULBP-1;ALCAN-β;N2DL1; MULT1;ALCAN-b
Accession
Species
 Human
Biotinylated
 yes
Amino Acid Range
 Gly26-Pro215
Molecular Weight
 The protein has a predicted MW of 25.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human NKG2D, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ULBP-1, His Tag with the EC50 of 0.14ug/ml determined by ELISA.
Product Tag
 C-His-Avi
Fluorophore
 Biotin-labeled
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in 20mM PB, 0.5M NaCl, 0.1M L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human NKG2D, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ULBP-1, His Tag with the EC50 of 0.14ug/ml determined by ELISA. Immobilized Human NKG2D, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ULBP-1, His Tag with the EC50 of 0.14ug/ml determined by ELISA.
Biotinylated Human ULBP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human ULBP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human ULBP-1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human ULBP-1 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...