Biotinylated Human ULBP-1 Protein (ULB-HM4P1B)
Biotinylated Human ULBP-1 Protein (ULB-HM4P1B)
Biotinylated Human ULBP-1 Protein (ULB-HM4P1B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human NKG2D, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ULBP-1, His Tag with the EC50 of 0.14ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gly26-Pro215
Catalog #ULB-HM4P1B
Regular price $525.00
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Product Description

Recombinant Biotinylated Human ULBP-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly26-Pro215.

Product Background

Cell surface proteins major histocompatibility complex (MHC) class I-related chain A (MICA) and UL16-binding proteins (ULBP) 1, 2, and 3 are up-regulated upon infection or tumor transformation and can activate human natural killer (NK) cells.

Product Category

Recombinant Protein

Protein

ULBP-1

Synonyms

ALCAN-beta; NKG2D ligand 1; N2DL-1; NKG2DL1; MULT-1; NKG2DL; AET1I; ULBP1; ULBP-1;ALCAN-β

Accession

Species

Human

Biotinylated

yes

Amino Acid Range

Gly26-Pro215

Molecular Weight

The protein has a predicted MW of 25.2 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human NKG2D, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ULBP-1, His Tag with the EC50 of 0.14ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 20mM PB, 0.5M NaCl, 0.1M L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human NKG2D, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ULBP-1, His Tag with the EC50 of 0.14ug/ml determined by ELISA. Immobilized Human NKG2D, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human ULBP-1, His Tag with the EC50 of 0.14ug/ml determined by ELISA.
Biotinylated Human ULBP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human ULBP-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Human ULBP-1 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human ULBP-1 is greater than 95% as determined by SEC-HPLC.

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