Cynomolgus CA9/Carbonic Anhydrase IX Protein (CA9-CM101)
Cynomolgus CA9/Carbonic Anhydrase IX Protein (CA9-CM101)
Cynomolgus CA9/Carbonic Anhydrase IX Protein (CA9-CM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus CA9, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gln38-Asp402
Catalog #CA9-CM101
Regular price $315.00
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Product Description

Recombinant Cynomolgus CA9/Carbonic Anhydrase IX Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln38-Asp402.

Product Background

CA9 is a member of the carbonic anhydrases' family, that is often expressed in cancer cells under hypoxic condition. CA9 expression potentially contributes to the regulation of cancer cell differentiation and mediates tumour-associated genes and signalling pathways, including apoptosis, hypoxia, G2M checkpoint, PI3K/AKR/mTOR signalling and TGF-beta signalling pathways.

Product Category

Recombinant Protein / Antibody discovery

Protein

CA9/Carbonic Anhydrase IX

Synonyms

CA9; CAIX; CA-IX; EC 4.2.1.1; G250; MN; P54/58N; PMW1; RCC

Accession

Species

Cynomolgus

Biotinylated

no

Amino Acid Range

Gln38-Asp402

Molecular Weight

The protein has a predicted MW of 41.03 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Cynomolgus CA9, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Cynomolgus CA9, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA (QC Test). Immobilized Cynomolgus CA9, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 9.3ng/ml determined by ELISA (QC Test).
Cynomolgus CA9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Cynomolgus CA9 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Cynomolgus CA9 is greater than 95% as determined by SEC-HPLC. The purity of Cynomolgus CA9 is greater than 95% as determined by SEC-HPLC.

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