Human CD5 Protein (CD5-HM501)

  • Express System: Expi293
  • Product Tag: C-hFc-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human CD5 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD5 Antibody, hFc Tag with the EC50 19.6ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Arg25-Asn371

Catalog: CD5-HM501

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Product Description Recombinant Human CD5 Protein is expressed from Expi293 with hFc tag and Avi tag at the C-terminal.; It contains Arg25-Asn371.
Product Category Recombinant Protein
Target CD5
Synonyms CD5 molecule; CD5; LEU1; T1; CD5 antigen
Gene/Protein ID P06127
Species Human
Exact Sequence Arg25-Asn371
Molecular Weight The protein has a predicted MW of 67.1 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD5 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD5 Antibody, hFc Tag with the EC50 19.6ng/ml determined by ELISA. See testing image for detail.
Product Tag C-hFc-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD5 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-CD5 Antibody, hFc Tag with the EC50 19.6ng/ml determined by ELISA.
The purity of Human CD5 is greater than 95% as determined by SEC-HPLC.
Human CD5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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