Product Description |
Recombinant Human CD5 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Arg25-Asn371. |
Product Background |
CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma. |
Product Category |
Recombinant Protein |
Target |
CD5 |
Synonyms |
CD5 molecule; CD5; LEU1; T1; CD5 antigen |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Arg25-Asn371 |
Molecular Weight |
The protein has a predicted MW of 67.1 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD5 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD5 Antibody, hFc Tag with the EC50 24.0ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-hFc-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human CD5 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD5 Antibody, hFc Tag with the EC50 24.0ng/ml determined by ELISA (QC Test). |
|
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Human CD5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human CD5 is greater than 95% as determined by SEC-HPLC. |
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