Human CD7 Protein (CD7-HM401)
Human CD7 Protein (CD7-HM401)
Human CD7 Protein (CD7-HM401)
Human CD7 Protein (CD7-HM401)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SECTM1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Ala26-Pro180
Catalog #CD7-HM401
Regular price $330.00
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Product Description

Recombinant Human CD7 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala26-Pro180.

Product Background

CD7, also known as Leu-9, is an approximately 40 kDa glycosylated and palmitoylated transmembrane protein in the immunoglobulin superfamily.CD7 is expressed on T cells, NK cells , myeloid progenitor cells, and CD19 B progenitor cells. Among CD8 T cells, the CD7-bright population preferentially contains naïve and memory cells, while more weak expressors are primarily effector cells.

Product Category

Recombinant Protein / Antibody discovery / CAR-T targets

Protein

CD7

Synonyms

CD7 molecule; CD7; LEU-9; Tp40; TP41; GP40

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ala26-Pro180

Molecular Weight

The protein has a predicted MW of 19.3 kDa. Due to glycosylation, the protein migrates to 32-45 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human CD7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SECTM1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human CD7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SECTM1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test). Immobilized Human CD7, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human SECTM1, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).
Immobilized Human CD7, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD7 Antibody, hFc Tag with the EC50 3.2ng/ml determined by ELISA. Immobilized Human CD7, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD7 Antibody, hFc Tag with the EC50 3.2ng/ml determined by ELISA.
Human CD7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD7 is greater than 95% as determined by SEC-HPLC. The purity of Human CD7 is greater than 95% as determined by SEC-HPLC.

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