Human CD83 Protein (CD8-HM283)
Human CD83 Protein (CD8-HM283)
Human CD83 Protein (CD8-HM283)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD83, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD83 Antibody, hFc Tag with the EC50 36.9ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Thr20-Ala143
Catalog #CD8-HM283
Regular price $195.00
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Product Description

Recombinant Human CD83 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Thr20-Ala143.

Product Background

CD83 is a member of the immunoglobulin (Ig) superfamily and is expressed in membrane bound or soluble forms. Membrane CD83 (mCD83) can be detected on a variety of activated immune cells, although it is most highly and stably expressed by mature dendritic cells (DC). While CD83 is emerging as a promising immune modulator with therapeutic potential, some important aspects such as its ligand/s, intracellular signaling pathways and modulators of its expression are unclear.

Product Category

Recombinant Protein / Antibody discovery

Protein

CD83

Synonyms

CD83; HB15; BL11; CD83 molecule

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Thr20-Ala143

Molecular Weight

The protein has a predicted MW of 40.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human CD83, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD83 Antibody, hFc Tag with the EC50 36.9ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human CD83, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD83 Antibody, hFc Tag with the EC50 36.9ng/ml determined by ELISA (QC Test). Immobilized Human CD83, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD83 Antibody, hFc Tag with the EC50 36.9ng/ml determined by ELISA (QC Test).
Human CD83 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD83 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD83 is greater than 95% as determined by SEC-HPLC. The purity of Human CD83 is greater than 95% as determined by SEC-HPLC.

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