Name: Human CD83 Protein (CD8-HM283)
Brand: KactusBio
SKU: CD8-HM283-100UG
Price: 214.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD83, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD83 Antibody, hFc Tag with the EC50 25.3ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Thr20-Ala143

Catalog #CD8-HM283

Size

100UG

500UG

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Human CD83 Protein (CD8-HM283) Summary

Product Description	Recombinant Human CD83 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Thr20-Ala143.
Product Background	CD83 is a member of the immunoglobulin (Ig) superfamily and is expressed in membrane bound or soluble forms. Membrane CD83 (mCD83) can be detected on a variety of activated immune cells, although it is most highly and stably expressed by mature dendritic cells (DC). While CD83 is emerging as a promising immune modulator with therapeutic potential, some important aspects such as its ligand/s, intracellular signaling pathways and modulators of its expression are unclear.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human CD83 Protein (CD8-HM283) Specifications/Details

Protein	CD83
Synonyms	CD83; HB15; BL11; CD83 molecule
Accession	Q01151-1
Species	Human
Biotinylated	no
Amino Acid Range	Thr20-Ala143
Molecular Weight	The protein has a predicted MW of 40.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD83, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD83 Antibody, hFc Tag with the EC50 25.3ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human CD83, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD83 Antibody, hFc Tag with the EC50 25.3ng/ml determined by ELISA (QC Test).
	Human CD83 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CD83 is greater than 95% as determined by SEC-HPLC.