Human DKK1 Protein (DKK-HM101)
Human DKK1 Protein (DKK-HM101)
Human DKK1 Protein (DKK-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human DKK1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.63ug/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Thr32-His266
Catalog #DKK-HM101
Regular price $181.50
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Product Description

Recombinant Human DKK1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr32-His266.

Product Background

Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy. Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains separated by a linker region. Dkk antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
DKK1
Synonyms
Dickkopf-1; Dkk1; SK
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Thr32-His266
Molecular Weight
The protein has a predicted MW of 26.87 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human DKK1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.63ug/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human DKK1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.63ug/ml determined by ELISA (QC Test). Immobilized Human DKK1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-5, mFc Tag with the EC50 of 0.63ug/ml determined by ELISA (QC Test).
Immobilized Human DKK1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 1.03ug/ml determined by ELISA. Immobilized Human DKK1, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human LRP-6, mFc Tag with the EC50 of 1.03ug/ml determined by ELISA.
Immobilized Human DKK1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. Immobilized Human DKK1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-DKK1 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
Human DKK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human DKK1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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