Name: Human EPO/Erythropoietin-Protein (EPO-HM001)
Brand: KactusBio
SKU: EPO-HM001-100UG
Price: 198.0 USD
Availability: InStock

Human EPO/Erythropoietin-Protein (EPO-HM001)

Immobilized Human EPO, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA.

Human EPO on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50-for this effect is 0.4 ‑ 1 ng/mL (QC Test).

The purity of Human EPO is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human EPO, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Ala28-Arg193

Catalog #EPO-HM001

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human EPO/Erythropoietin-Protein (EPO-HM001) Summary

Product Description	Recombinant Human EPO/Erythropoietin-Protein is expressed from HEK293 without tag.It contains Ala28-Arg193.
Product Background	Erythropoietin (EPO) is a circulating hormone conventionally considered to be responsible for erythropoiesis. In addition to facilitating red blood cell production, EPO has pluripotent potential, such as for cognition improvement, neurogenesis, and anti-fibrotic, anti-apoptotic, anti-oxidative, and anti-inflammatory effects.
Product Category	Recombinant Protein / Cell therapy / Cell culture / Cytokines

Human EPO/Erythropoietin-Protein (EPO-HM001) Specifications/Details

Protein	EPO/Erythropoietin
Synonyms	EPO; Erythropoietin; EP; MVCD2; Epoetin
Accession	AAH93628.1
Species	Human
Biotinylated	no
Amino Acid Range	Ala28-Arg193
Molecular Weight	The protein has a predicted MW of 18.39 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human EPO, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA. See testing image for detail.
Product Tag	No Tag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human EPO, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human EPO R, hFc Tag with the EC50 of 29.7ng/ml determined by ELISA.
	Human EPO on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50-for this effect is 0.4 ‑ 1 ng/mL (QC Test).
	The purity of Human EPO is greater than 95% as determined by SEC-HPLC.