Name: Human GDF-2/BMP-9 Protein, Ultra Low Endotoxin (GDF-HM002-UL)
Brand: KactusBio
SKU: GDF-HM002-UL-100UG
Price: 472.0 USD
Availability: InStock

Human GDF-2/BMP-9 Protein, Ultra Low Endotoxin (GDF-HM002-UL)

Human/Cynomolgus Activin RIIB, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF-2, No Tag with an affinity constant of 26.57 pM as determined in SPR assay (Biacore T200).

Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test).

Immobilized Human GDF-2, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GDF-2 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA.

Human GDF-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50-for this effect is 0.4-4 ng/mL (QC Test).

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test). The affinity constant of 26.57 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ser320-Arg429

Catalog #GDF-HM002-UL

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human GDF-2/BMP-9 Protein, Ultra Low Endotoxin (GDF-HM002-UL) Summary

Product Description	Recombinant Human GDF-2/BMP-9 Protein is expressed from HEK293 without tag.It contains Ser320-Arg429.
Product Background	Bone morphogenetic proteins (BMPs) are expressed in different cell types of the human ovarian follicle and play important roles in the regulation of ovarian function. BMP-9, also known as growth differentiation factor-2 (GDF-2), belongs to the transforming growth factor-beta (TGF-β) superfamily. BMP-9 is mainly synthesized in the liver and secreted into the blood which allows it to regulate various physiological and pathological functions.
Product Category	Recombinant Protein / Cell therapy / Cell culture / Cytokines

Human GDF-2/BMP-9 Protein, Ultra Low Endotoxin (GDF-HM002-UL) Specifications/Details

Protein	GDF-2/BMP-9
Synonyms	GDF-2; GDF2; BMP-9; BMP9
Accession	Q9UK05
Species	Human
Biotinylated	no
Amino Acid Range	Ser320-Arg429
Molecular Weight	The protein has a predicted MW of 12.08 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test). The affinity constant of 26.57 pM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 0.001 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 4mM HCL. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in 4mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Human/Cynomolgus Activin RIIB, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF-2, No Tag with an affinity constant of 26.57 pM as determined in SPR assay (Biacore T200).
	Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test).
	Immobilized Human GDF-2, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GDF-2 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA.
	Human GDF-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50-for this effect is 0.4-4 ng/mL (QC Test).