Product Description |
Recombinant Human GDF-2/BMP-9 Protein is expressed from HEK293 without tag.It contains Ser320-Arg429. |
Product Background |
Bone morphogenetic proteins (BMPs) are expressed in different cell types of the human ovarian follicle and play important roles in the regulation of ovarian function. BMP-9, also known as growth differentiation factor-2 (GDF-2), belongs to the transforming growth factor-beta (TGF-β) superfamily. BMP-9 is mainly synthesized in the liver and secreted into the blood which allows it to regulate various physiological and pathological functions. |
Product Category |
Recombinant Protein / Cell therapy / Cell culture / Cytokines |
Protein |
GDF-2/BMP-9 |
Synonyms |
GDF-2; GDF2; BMP-9; BMP9 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser320-Arg429 |
Molecular Weight |
The protein has a predicted MW of 12.08 kDa. Due to glycosylation, the protein migrates to 13-15 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test). The affinity constant of 26.57 pM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
No Tag |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.001 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 4mM HCL. Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in 4mM HCL. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Human/Cynomolgus Activin RIIB, hFc Tag captured on CM5 Chip via Protein A can bind Human GDF-2, No Tag with an affinity constant of 26.57 pM as determined in SPR assay (Biacore T200). |
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Immobilized Human GDF-2, No Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ALK-1, His Tag with the EC50 of 33.1ng/ml determined by ELISA (QC Test). |
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Immobilized Human GDF-2, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-GDF-2 Antibody, hFc Tag with the EC50 of 5.5ng/ml determined by ELISA. |
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Human GDF-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50-for this effect is 0.4-4 ng/mL (QC Test). |
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