Name: Non-biotinylated Human Fc gamma RIIA/CD32a (H167) Protein (CDA-HM432)
Brand: KactusBio
SKU: CDA-HM432-100UG
Price: 264.0 USD
Availability: InStock

Non-biotinylated Human Fc gamma RIIA/CD32a (H167) Protein (CDA-HM432)

Human Fc gamma RIIA (H167) , His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 1.53 uM as determined in SPR assay (Biacore T200) (QC Test).

Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIA (H167) , His Tag with an affinity constant of 0.73 uM as determined in SPR assay (Biacore T200).

Human Fc gamma RIIA (H167) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Human Fc gamma RIIA (H167), His Tag on Anti-His-Biosensor, can bind Rituximab with an affinity constant of 0.71 µM as determined in BLI assay (Gator).

The purity of-Human Fc gamma RIIA (H167) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 1.53 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ala36-Ile218(H167)

Catalog #CDA-HM432

Size

100UG

500UG

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Product Description	Recombinant Human Fc gamma RIIA/CD32a (H167) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ala36-Ile218(H167).
Product Background	The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type.
Product Category	Recombinant Protein / Antibody discovery / Fc receptors

Protein	Fc gamma RIIA/CD32a (H167)
Synonyms	FcgammaRIIA; Fc gammaRIIA; Fcgamma RIIA; Fc gamma RIIA; FcγRIIA; Fc γRIIA; Fcγ RIIA; Fc γ RIIA; FcgRIIA; Fc gRIIA; Fcg RIIA; Fc g RIIA; FCG2; FCGR2; FCGR2A; FCGR2A1; FCRIIA; fcRII-a; FCG2; CD32A
Accession	P12318-1
Species	Human
Biotinylated	no
Amino Acid Range	Ala36-Ile218(H167)
Molecular Weight	The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity	The affinity constant of 1.53 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human Fc gamma RIIA (H167) , His Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 1.53 uM as determined in SPR assay (Biacore T200) (QC Test).
	Rituximab captured on CM5 Chip via Protein A can bind Human Fc gamma RIIA (H167) , His Tag with an affinity constant of 0.73 uM as determined in SPR assay (Biacore T200).
	Human Fc gamma RIIA (H167) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Human Fc gamma RIIA (H167), His Tag on Anti-His-Biosensor, can bind Rituximab with an affinity constant of 0.71 µM as determined in BLI assay (Gator).
	The purity of-Human Fc gamma RIIA (H167) is greater than 95% as determined by SEC-HPLC.

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