Product Description |
Recombinant Human FGFR1 alpha (IIIc) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg22-Glu374. |
Product Background |
Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding.FGFR1 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system. |
Product Category |
Recombinant Protein |
Target |
FGFR1 alpha (IIIc) |
Synonyms |
FGF R1a; FGFR1 alpha |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Arg22-Glu374 |
Molecular Weight |
The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 68-83 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human FGFR1 alpha (IIIc) at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test). The affinity constant of 42.45 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human FGFR1 alpha (IIIc), His Tag captured on CM5 Chip via Anti-His Antibody can bind Human FGF1, No tag with an affinity constant of 42.45 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human FGFR1 alpha (IIIc) at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test). |
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|
Human FGFR1 alpha (IIIc) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. |
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The purity of Human FGFR1 alpha (IIIc) is greater than 95% as determined by SEC-HPLC. |
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