Human FGFR1 alpha (IIIc) Protein (FGF-HM4RA)
Human FGFR1 alpha (IIIc) Protein (FGF-HM4RA)
Human FGFR1 alpha (IIIc) Protein (FGF-HM4RA)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FGFR1 alpha (IIIc) at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test). The affinity constant of 42.45 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Arg22-Glu374
Catalog #FGF-HM4RA
Regular price $150.00
/
Size

Order now, ships by:

Product Description

Recombinant Human FGFR1 alpha (IIIc) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg22-Glu374.

Product Background

Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding.FGFR1 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.

Product Category

Recombinant Protein / Antibody discovery

Protein

FGFR1 alpha (IIIc)

Synonyms

FGF R1a; FGFR1 alpha

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Arg22-Glu374

Molecular Weight

The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 68-83 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human FGFR1 alpha (IIIc) at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test). The affinity constant of 42.45 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human FGFR1 alpha (IIIc), His Tag captured on CM5 Chip via Anti-His Antibody can bind Human FGF1, No tag with an affinity constant of 42.45 nM as determined in SPR assay (Biacore T200). Human FGFR1 alpha (IIIc), His Tag captured on CM5 Chip via Anti-His Antibody can bind Human FGF1, No tag with an affinity constant of 42.45 nM as determined in SPR assay (Biacore T200).
Immobilized Human FGFR1 alpha (IIIc) at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test). Immobilized Human FGFR1 alpha (IIIc) at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).
Immobilized Human FGFR1 alpha (IIIc), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi Tag with the EC50 of 60.0ng/ml determined by ELISA. Immobilized Human FGFR1 alpha (IIIc), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi Tag with the EC50 of 60.0ng/ml determined by ELISA.
Human FGFR1 alpha (IIIc) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%. Human FGFR1 alpha (IIIc) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human FGFR1 alpha (IIIc) is greater than 95% as determined by SEC-HPLC. The purity of Human FGFR1 alpha (IIIc) is greater than 95% as determined by SEC-HPLC.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed