Name: Human FGFR1 alpha (IIIc) Protein (FGF-HM4RA)
Brand: KactusBio
SKU: FGF-HM4RA-100UG
Price: 150.0 USD
Availability: InStock

Human FGFR1 alpha (IIIc) Protein (FGF-HM4RA)

Human FGFR1 alpha (IIIc), His Tag captured on CM5 Chip via Anti-His Antibody can bind Human FGF1, No tag with an affinity constant of 42.45 nM as determined in SPR assay (Biacore T200).

Immobilized Human FGFR1 alpha (IIIc) at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).

Immobilized Human FGFR1 alpha (IIIc), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi Tag with the EC50 of 60.0ng/ml determined by ELISA.

Human FGFR1 alpha (IIIc) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.

The purity of Human FGFR1 alpha (IIIc) is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human FGFR1 alpha (IIIc) at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test). The affinity constant of 42.45 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Arg22-Glu374

Catalog #FGF-HM4RA

Size

100UG

500UG

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Product Description	Recombinant Human FGFR1 alpha (IIIc) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Arg22-Glu374.
Product Background	Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding.FGFR1 is tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of embryonic development, cell proliferation, differentiation and migration. Required for normal mesoderm patterning and correct axial organization during embryonic development, normal skeletogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.
Product Category	Recombinant Protein / Antibody discovery

Protein	FGFR1 alpha (IIIc)
Synonyms	FGF R1a; FGFR1 alpha
Accession	P11362-7
Species	Human
Biotinylated	no
Amino Acid Range	Arg22-Glu374
Molecular Weight	The protein has a predicted MW of 42 kDa. Due to glycosylation, the protein migrates to 68-83 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human FGFR1 alpha (IIIc) at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test). The affinity constant of 42.45 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human FGFR1 alpha (IIIc), His Tag captured on CM5 Chip via Anti-His Antibody can bind Human FGF1, No tag with an affinity constant of 42.45 nM as determined in SPR assay (Biacore T200).
	Immobilized Human FGFR1 alpha (IIIc) at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-FGFR1 Antibody, hFc Tag with the EC50 of 4.6ng/ml determined by ELISA (QC Test).
	Immobilized Human FGFR1 alpha (IIIc), His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human FGF1, His-Avi Tag with the EC50 of 60.0ng/ml determined by ELISA.
	Human FGFR1 alpha (IIIc) on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
	The purity of Human FGFR1 alpha (IIIc) is greater than 95% as determined by SEC-HPLC.