Product Description |
Recombinant Human Fibronectin (1266-1356) Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Glu1266-Thr1356. |
Product Background |
Fibronectin is a high molecular glycoprotein present in the blood, connective tissue and at cell surface. It is synthesized by many types of differentiated cells and is believed to be involved in the attachment of cells to the surrounding extracellular matrix. Fibronectin has affinity to the other main components of extracellular matrix, collagen and glycosaminoglycans. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
Fibronectin (1266-1356) |
Synonyms |
FN; CIG; FN1; ED-B; FINC; FNZ; GFND; GFND2; LETS; MSF; SMDCF |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu1266-Thr1356 |
Molecular Weight |
The protein has a predicted MW of 36.47 kDa. Due to glycosylation, the protein migrates to 47-57 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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