Human Her3/ErbB3 Protein (HER-HM403)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Her3, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Her3 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Ser20-Thr643

Catalog: HER-HM403

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Product Description Recombinant Human Her3/ErbB3 is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser20-Thr643.

Product Background

Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors.Her3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric Her3 serves as a low affinity receptor for the heregulins (HRG).

Product Category

Recombinant Protein

Target

Her3/ErbB3
Synonyms ErbB3; ErbB-3; HER3; HER3c-erbB-3; LCCS2; MDA-BF-1; MGC88033; p180-ErbB3; p45-sErbB3; p85-sErbB3; ERBB3

Gene/Protein ID

P21860-1

Species

Human

Exact Sequence

Ser20-Thr643
Molecular Weight The protein has a predicted MW of 71.6 kDa. Due to glycosylation, the protein migrates to 72-75 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human Her3, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Her3 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Her3, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Her3 Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test).
Immobilized Human Her3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human NRG1 Beta 1, hFc Tag with the EC50 of 48.6ng/ml determined by ELISA.
The purity of Human Her3 is greater than 95% as determined by SEC-HPLC.
Human Her3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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