Human IL-12 R beta 1/CD212 Protein (ILR-HM112)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 98.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Cys24-Glu540
Catalog #ILR-HM112
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Product Description
 Recombinant Human IL-12 R beta 1/CD212 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys24-Glu540.
Product Background
 Human IL12RB1 is an autosomal gene that is essential for mycobacterial disease resistance and T cell differentiation. Lung and T cell IL12RB1 expression is allele-biased, and the extent to which cells express one IL12RB1 allele is unaffected by activation. Furthermore following its expression the IL12RB1 pre-mRNA is processed into either IL12RB1 Isoform 1 (IL12Rβ1, a positive regulator of IL12 responsiveness) or IL12RB1 Isoform 2 (a protein of heretofore unknown function).
Product Category
 Recombinant Protein
Target
 IL-12 R beta 1/CD212
Synonyms
 IL-12R-beta-1; IL-12RB1; IL12R; IL12RB; CD212; IL-12R subunit beta-1; IL-12R-BETA1; MGC34454
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Cys24-Glu540
Molecular Weight
 The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 98.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-12B, His Tag immobilized on CM5 Chip can bind Human IL-12 R beta 1, His Tag with an affinity constant of 98.1nM as determined in a SPR assay (Biacore T200).
Human IL-12 R beta 1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-12 R beta 1 is greater than 95% as determined by SEC-HPLC.

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