Human IL-12 R beta 1/CD212 Protein (ILR-HM112)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 98.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Cys24-Glu540

Catalog: ILR-HM112

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Product Description	Recombinant Human IL-12 R beta 1/CD212 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys24-Glu540.
Product Background	Human IL12RB1 is an autosomal gene that is essential for mycobacterial disease resistance and T cell differentiation. Lung and T cell IL12RB1 expression is allele-biased, and the extent to which cells express one IL12RB1 allele is unaffected by activation. Furthermore following its expression the IL12RB1 pre-mRNA is processed into either IL12RB1 Isoform 1 (IL12Rβ1, a positive regulator of IL12 responsiveness) or IL12RB1 Isoform 2 (a protein of heretofore unknown function).
Product Category	Recombinant Protein

Target	IL-12 R beta 1/CD212
Synonyms	IL-12R-beta-1; IL-12RB1; IL12R; IL12RB; CD212; IL-12R subunit beta-1; IL-12R-BETA1; MGC34454
Gene/Protein ID	P42701-1
Species	Human
Exact Sequence	Cys24-Glu540
Molecular Weight	The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 98.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-12B, His Tag immobilized on CM5 Chip can bind Human IL-12 R beta 1, His Tag with an affinity constant of 98.1nM as determined in a SPR assay (Biacore T200).
	The purity of Human IL-12 R beta 1 is greater than 95% as determined by SEC-HPLC.
	Human IL-12 R beta 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.