Product Description |
Recombinant Human IL-13 Protein is expressed from HEK293 without tag.It contains Gly21-Asn132. |
Product Background |
Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity). |
Product Category |
Recombinant Protein / Cell therapy / Cell culture / Cytokines |
Protein |
IL-13 |
Synonyms |
IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly21-Asn132 |
Molecular Weight |
The protein has a predicted MW of 12.35 kDa. Due to glycosylation, the protein migrates to 20-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-13, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 16.2ng/ml determined by ELISA. The affinity constant of 0.55 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
No Tag |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Human IL-13, No Tag immobilized on CM5 Chip can bind Human IL-13Ra1, His Tag with an affinity constant of 0.55 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human IL-13, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, His Tag with the EC50 of 16.2ng/ml determined by ELISA. |
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Human IL-13 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is typically 5 - 40ng/mL (QC Test). |
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The purity of Human IL-13 is greater than 95% as determined by SEC-HPLC. |
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