Human IL-13 Protein (IL1-HM413)

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 90% as determined by Tris-Bis PAGE
Activity: Immobilized Human IL-13, His Tag at 5ug/ml (100ul/Well). Dose response curve for Human IL-13Ra1, hFc Tag with the EC50 of 4.7ug/ml determined by ELISA. The affinity constant of 0.7uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gly21-Asn132

Catalog: IL1-HM413

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Product Description	Recombinant Human IL-13 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gly21-Asn132.
Product Background	Interleukin-13 (IL-13) is a monomeric 17 kDa immunoregulatory cytokine that plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages (By similarity).
Product Category	Recombinant Protein

Target	IL-13
Synonyms	IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789
Gene/Protein ID	AAK53823
Species	Human
Exact Sequence	Gly21-Asn132
Molecular Weight	The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 15 kDa and 25-45 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-13, His Tag at 5ug/ml (100ul/Well). Dose response curve for Human IL-13Ra1, hFc Tag with the EC50 of 4.7ug/ml determined by ELISA. The affinity constant of 0.7uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Avi
Express System	HEK293
Purity	> 90% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-13, His Tag immobilized on CM5 Chip can bind Human IL-13Ra1, hFc Tag with an affinity constant of 0.7uM as determined in a SPR assay (Biacore T200).
	Immobilized Human IL-13, His Tag at 5ug/ml (100ul/Well). Dose response curve for Human IL-13Ra1, hFc Tag with the EC50 of 4.7ug/ml determined by ELISA.
	Immobilized Human IL-13, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 1.0ug/ml determined by ELISA (QC Test).
	Human IL-13 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.