Human IL-13 Protein (IL1-HM413)
Catalog: IL1-HM413
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| Product Description | Recombinant Human IL-13 Protein is expressed from Expi293 with His tag and Avi tag at the N-terminal.It contains Gly21-Asn132. |
| Product Category | Recombinant Protein |
| Target | IL-13 |
| Synonyms | IL13; IL-13; interleukin 13; NC30; P600; ALRH; BHR1; MGC116786; MGC116788; MGC116789 |
| Gene/Protein ID | AAK53823 |
| Species | Human |
| Exact Sequence | Gly21-Asn132 |
| Molecular Weight | The protein has a predicted MW of 15.3 kDa. Due to glycosylation, the protein migrates to 15 kDa and 25-45 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human IL-13, His Tag at 1μg/ml (100μl/Well). Dose response curve for Human IL-13Ra1, hFc Tag with the EC50 of 2.7μg/ml determined by ELISA. The affinity constant of 0.7μM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | N-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human IL-13, His Tag immobilized on CM5 Chip can bind Human IL-13Ra1, hFc Tag with an affinity constant of 0.7μM as determined in a SPR assay (Biacore T200). |
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Immobilized Human IL-13, His Tag at 1μg/ml (100μl/Well). Dose response curve for Human IL-13Ra1, hFc Tag with the EC50 of 2.7μg/ml determined by ELISA. |
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Immobilized Human IL-13, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human IL-13Ra2, hFc Tag with the EC50 of 2.6μg/ml determined by ELISA. |
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The purity of Human IL-13 is greater than 95% as determined by SEC-HPLC. |
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Human IL-13 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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