Product Description |
Recombinant Human IL-27RA/TCCR Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly34-Lys516. |
Product Background |
A multicenter, web-based Thyroid Cancer and Tumor Collaborative Registry (TCCR, http://tccr.unmc.edu) allows for the collection and management of various data on thyroid cancer (TC) and thyroid nodule (TN) patients. The TCCR is coupled with OpenSpecimen, an open-source biobank management system, to annotate biospecimens obtained from the TCCR subjects. |
Product Category |
Recombinant Protein |
Protein |
IL-27 Ra/TCCR |
Synonyms |
IL-27RA; TCCR; IL27RA; IL-27R-alpha; IL27R alpha; WSX-1; WSX1; ZcytoR1; CRL1; IL-27 RA; IL27 RA |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly34-Lys516 |
Molecular Weight |
The protein has a predicted MW of 79.3 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-27 RA, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test). The affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS, 5mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human IL-27 RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-27, His Tag with an affinity constant of 3.58 nM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Human IL-27 RA, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IL-27, His Tag with the EC50 of 55.9ng/ml determined by ELISA (QC Test). |
|
|
Human IL-27RA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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