Name: Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein (MHC-HM418T)
Brand: KactusBio
SKU: MHC-HM418T-100UG
Price: 750.0 USD
Availability: InStock

Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein (MHC-HM418T)

Anti-HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody captured on CM5 Chip via Protein A can bind Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer, His Tag with an affinity constant of 42.3 nM as determined in SPR assay (Biacore T200).

Immobilized Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody, hFc Tag with the EC50 of 0.18ug/ml determined by ELISA (QC Test).

Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.

The purity of Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody, hFc Tag with the EC50 of 0.18ug/ml determined by ELISA (QC Test). The affinity constant of 42.3 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and VVVGAVGVGK peptide

Catalog #MHC-HM418T

Size

100UG

500UG

1MG

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Product Description	Recombinant Human HLA-A03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A03:01), Ile21-Met119(B2M) and VVVGAVGVGK peptide.
Product Background	Kirsten rat sarcoma 2 viral oncogene homolog (KRAS) is the most commonly mutated oncogene in human cancer. The developments of many cancers depend on sustained expression and signaling of KRAS, which makes KRAS a high-priority therapeutic target. The virtual screening approach to discover novel KRAS inhibitors and synthetic lethality interactors of KRAS are discussed in detail.
Product Category	Recombinant Protein / Antibody discovery / MHC

Protein	KRAS G12V (HLA-A*03:01)
Synonyms	MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
MHC Form	Tetramer
Allele	HLA-A*03:01
Accession	NP_002107.3(HLA-A*03:01)&P61769(B2M)&VVVGAVGVGK
Species	Human
Biotinylated	no
Amino Acid Range	Gly25-Thr305(HLA-A*03:01), Ile21-Met119(B2M) and VVVGAVGVGK peptide
Molecular Weight	The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Bis-Tris PAGE result.
Activity	Immobilized Human HLA-A03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody, hFc Tag with the EC50 of 0.18ug/ml determined by ELISA (QC Test). The affinity constant of 42.3 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Anti-HLA-A03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody captured on CM5 Chip via Protein A can bind Human HLA-A03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer, His Tag with an affinity constant of 42.3 nM as determined in SPR assay (Biacore T200).
	Immobilized Human HLA-A03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A03:01&B2M&KRAS G12V (VVVGAVGVGK) Antibody, hFc Tag with the EC50 of 0.18ug/ml determined by ELISA (QC Test).
	Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer on Bis-Tris PAGE under Non reducing (N) condition. The purity is greater than 95%.
	The purity of Human HLA-A*03:01&B2M&KRAS G12V (VVVGAVGVGK) Tetramer is greater than 95% as determined by SEC-HPLC.