Human KRAS G12V (HLA-A*03:01) Tetramer Protein (MHC-HM418T)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human KRAS G12V (HLA-A*03:01) Tetramer, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-KRAS G12V (HLA-A*03:01) Antibody, hFc Tag with the EC50 of 0.58μg/ml determined by ELISA. The affinity constant of 42.3 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M)and VVVGAVGVGK peptide

Catalog: MHC-HM418T

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Product Description Recombinant Human KRAS G12V (HLA-A*03:01) Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M) and VVVGAVGVGK peptide.
Product Category Recombinant Protein
Target KRAS G12V (HLA-A*03:01)
Synonyms MHC; KRAS; K-Ras 2; KRAS2; C-K-RAS; CFC2; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KRAS1; KRAS2; NS; NS3; RASK2; GTPase Kras; KI-RAS; RALD
Gene/Protein ID NP_002107.3(HLA-A*03:01)&P61769(B2M)&VVGAVGVGK
Species Human
Exact Sequence Gly25-Thr305(HLA-A*03:01),Ile21-Met119(B2M)and VVVGAVGVGK peptide
Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Activity Immobilized Human KRAS G12V (HLA-A*03:01) Tetramer, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-KRAS G12V (HLA-A*03:01) Antibody, hFc Tag with the EC50 of 0.58μg/ml determined by ELISA. The affinity constant of 42.3 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Anti-KRAS G12V (HLA-A*03:01) Antibody captured on CM5 Chip via Protein A can bind Human KRAS G12V (HLA-A*03:01) Tetramer, His Tag with an affinity constant of 42.3 nM as determined in SPR assay (Biacore T200).
Immobilized Human KRAS G12V (HLA-A*03:01) Tetramer, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-KRAS G12V (HLA-A*03:01) Antibody, hFc Tag with the EC50 of 0.58μg/ml determined by ELISA.
The purity of Human KRAS G12V (HLA-A*03:01) Tetramer is greater than 95% as determined by SEC-HPLC.
Human KRAS G12V (HLA-A*03:01) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

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