Human LAIR1/CD305 Protein (LAI-HM4R1)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LAIR1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LAIR1 Antibody, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA. The affinity constant of 0.082 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln22-His163

Catalog: LAI-HM4R1

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Product Description Recombinant Human LAIR1/CD305 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gln22-His163.
Product Category Recombinant Protein
Target LAIR1/CD305
Synonyms CD305; HLAIR1; LAIR1; LAIR-1
Gene/Protein ID Q6GTX8-1
Species Human
Exact Sequence Gln22-His163
Molecular Weight The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 30-36 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human LAIR1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LAIR1 Antibody, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA. The affinity constant of 0.082 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human LAIR1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Native Human Collagen I protein with an affinity constant of 0.082 nM as determined in SPR assay (Biacore T200).
Immobilized Human LAIR1, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-LAIR1 Antibody, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA.
The purity of Human LAIR1 is greater than 95% as determined by SEC-HPLC.
Human LAIR1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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