Human LAIR1/CD305 Protein (LAI-HM4R1)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LAIR1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LAIR1 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.082 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln22-His163

Catalog: LAI-HM4R1

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Product Description Recombinant Human LAIR1/CD305 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln22-His163.

Product Background

LAIR1 (leukocyte-associated Ig-like receptor-1, designated CD305) is an approximately 40 kDa type I transmembrane inhibitory glycoprotein belonging to the Ig superfamily.LAIR1 functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells. Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11. It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation.

Product Category

Recombinant Protein

Target

LAIR1/CD305
Synonyms CD305; HLAIR1; LAIR1; LAIR-1
Accession

Species

Human

Amino Acid Range

Gln22-His163
Molecular Weight The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 30-36 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human LAIR1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LAIR1 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). The affinity constant of 0.082 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human LAIR1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Native Human Collagen I protein with an affinity constant of 0.082 nM as determined in SPR assay (Biacore T200).
Immobilized Human LAIR1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-LAIR1 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).
The purity of Human LAIR1 is greater than 95% as determined by SEC-HPLC.
Human LAIR1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

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