Human Latent TGF beta 2/TGFB2 Protein (TGF-HM102)
Human Latent TGF beta 2/TGFB2 Protein (TGF-HM102)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 2.21 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Leu21-Ser414
Catalog #TGF-HM102
Regular price $405.00
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Product Description
Recombinant Human Latent TGF beta 2/TGFB2 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Leu21-Ser414.
Product Background
Transforming growth factor beta(2) (TGF-beta(2)), a growth regulator of human lens epithelial cells (HLECs), also regulates the death of these cells. TGF-beta(2)-induced apoptosis in HLECs was preceded by an induction of reactive oxygen species (ROS) and a decrease in glutathione in the intracellular content, indicating that this factor induces oxidative stress in HLECs.
Product Category
Recombinant Protein
Target
Latent TGF beta 2/TGFB2
Synonyms
TGFB2; TGFBR2; TGFR2; TbetaR-II; TGFβR2; BB105277; TGF-beta 2; TβR-II; TGF-β 2
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Leu21-Ser414
Molecular Weight
The protein has a predicted MW of 46.6 kDa. Due to glycosylation, the protein migrates to 40-60 kDa&12-13 kDa based on Bis-Tris PAGE result.
Activity
The affinity constant of 2.21 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
N-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TGF-beta RII, hFc Tag captured on CM5 Chip via Protein A can bind Human Latent TGF beta 2, His Tag with an affinity constant of 2.21 uM as determined in SPR assay (Biacore T200).
Human Latent TGF beta 2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Latent TGF beta 2 is greater than 95% as determined by SEC-HPLC.

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