Human LMP2 (HLA-A*02:01) Complex Tetramer Protein (MHC-HM411T)
Catalog: MHC-HM411T
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| Product Description | Recombinant Human LMP2 (HLA-A*02:01) Complex Tetramer Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal, tetramer is assembled by biotinylated monomer and streptavidin.; It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and CLGGLLTMV peptide. |
| Product Category | Recombinant Protein |
| Target | LMP2 (HLA-A*02:01) Complex Tetramer |
| Synonyms | MHC; RMF; LMP2; LMP-2; Macropain chain 7; Proteasome chain 7; PSMB9; RING12 |
| Gene/Protein ID | A0A140T913 (HLA-A*02:01)&P61769 (B2M)&CLGGLLTMV |
| Species | Human |
| Exact Sequence | Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and CLGGLLTMV peptide |
| Molecular Weight | The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. |
| Activity | Immobilized Human LMP2 (HLA-A*02:01) Complex Tetramer, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LMP2 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human LMP2 (HLA-A*02:01) Complex Tetramer, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Anti-LMP2 (HLA-A*02:01) Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA. |
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The purity of Human LMP2 (HLA-A*02:01) Complex Tetramer was greater than 95% as determined by SEC-HPLC. |
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Human LMP2 (HLA-A*02:01) Complex Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%. |
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