Human M-CSF/CSF-1 Protein (CSF-HM001)

Express System: HEK293
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human M-CSF at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 42.8ng/ml determined by ELISA (QC Test). The affinity constant of 2.06 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Glu33-Asn190

Catalog: CSF-HM001

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Product Description	Recombinant Human M-CSF/CSF-1 Protein is expressed from HEK293 without tag.It contains Glu33-Asn190.
Product Background	CSF-1, also known as M-CSF , is a four alpha -helical-bundle cytokine that is the primary regulator of macrophage survival, proliferation and differentiation. CSF-1 is also essential for the survival and proliferation of osteoclast progenitors.CSF-1 is cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. This mutation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
Product Category	Recombinant Protein

Target	M-CSF/CSF-1
Synonyms	CSF1; CSF-1; MCSF; M-CSF; MGC31930; Lanimostim
Gene/Protein ID	P09603-1
Species	Human
Exact Sequence	Glu33-Asn190
Molecular Weight	The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human M-CSF at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 42.8ng/ml determined by ELISA (QC Test). The affinity constant of 2.06 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human M-CSF, No Tag immobilized on CM5 Chip can bind Human M-CSF R, hFc Tag with an affinity constant of 2.06 nM as determined in SPR assay (Biacore T200).
	Immobilized Human M-CSF at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human M-CSF R, hFc Tag with the EC50 42.8ng/ml determined by ELISA (QC Test).
	Measured in a cell proliferation assay using RAW264.7 cells.The ED50-for this effect is 2.5 - 7.0 ng/mL (QC Test).
	The purity of Human M-CSF is greater than 95% as determined by SEC-HPLC.
	Human M-CSF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.