Product Description |
Recombinant Chimeric HLA-A*02:01(mα3)&B2M&LMP2 (CLGGLLTMV) Monomer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly25-Thr206(Human HLA-A*02:01 α1&α2)&Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(B2M) and CLGGLLTMV peptide. |
Product Background |
The immunoproteasome, having been linked to neurodegenerative diseases and hematological cancers, has been shown to play an important role in MHC class I antigen presentation. The development of molecular probes that selectively inhibit the major catalytic subunit, LMP2, of the immunoproteasome,LMP2-rich cancer cells compared to LMP2-deficient cancer cells are more sensitive to growth inhibition by the LMP2-specific inhibitor, implicating an important role of LMP2 in regulating cell growth of malignant tumors that highly express LMP2. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
Protein |
Chimeric HLA-A*02:01 (mα3) &B2M&LMP2 (CLGGLLTMV) |
Synonyms |
MHC; RMF; LMP2; LMP-2; Macropain chain 7; Proteasome chain 7; PSMB9; RING12 |
MHC Form |
Monomer |
Allele |
HLA-A*02:01 |
Accession |
|
Species |
Human&Mouse |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(B2M)and CLGGLLTMV peptide |
Molecular Weight |
The protein has a predicted MW of 50.3 kDa. Due to glycosylation, the protein migrates to 52-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Chimeric HLA-A*02:01(mα3)&B2M&LMP2 (CLGGLLTMV) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&LMP2 (CLGGLLTMV) Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Chimeric HLA-A*02:01(mα3)&B2M&LMP2 (CLGGLLTMV) Monomer, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&LMP2 (CLGGLLTMV) Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA. |
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Chimeric HLA-A*02:01 (mα3) &B2M&LMP2 (CLGGLLTMV) Monomer on Bis-Tris PAGE under reduced and non-reduced condition. The purity is greater than 95%. |
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The purity of Chimeric HLA-A*02:01 (mα3) &B2M&LMP2 (CLGGLLTMV) Monomer was greater than 95% as determined by SEC-HPLC. |
Additional Information
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