Product Description |
Recombinant Human Neuropilin-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe22-Lys644. |
Product Background |
Neuropilin 1 (NRP1) is a transmembrane glycoprotein that acts as a co-receptor for a number of extracellular ligands including class III/IV semaphorins, certain isoforms of vascular endothelial growth factor and transforming growth factor beta. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
Neuropilin-1 |
Synonyms |
BDCA4; NP1; Neuropilin-1; NRP1; CD304; VEGF165R; Neuropilin 1; NRP; Transmembrane Receptor; CD304 Antigen |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Phe22-Lys644 |
Molecular Weight |
The protein has a predicted MW of 70.97 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Neuropilin-1, His Tag with the EC50 of 0.57ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Neuropilin-1, His Tag with the EC50 of 0.57ug/ml determined by ELISA. |
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Human Neuropilin-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Neuropilin-1 is greater than 95% as determined by SEC-HPLC. |
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