Product Description |
Recombinant Human NGAL/Lipocalin-2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln21-Gly198. |
Product Background |
Acute kidney injury (AKI) is one of the most common complications of various serious conditions, and early diagnosis is therefore critical for the treatment of AKI. Recent evidence demonstrates that neutrophil gelatinase- associated lipocalin (NGAL) is closely associated with AKI. Several experimental and clinical studies have shown that the expression of urine and serum NGAL increases significantly in AKI. NGAL shows potential to be a new effective early biochemical marker of AKI. Further studies are needed to confirm the significant advantages of NGAL in the diagnosis of early AKI and its value in clinical applications. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
NGAL/Lipocalin-2 |
Synonyms |
NGAL; Lipocalin-2; Oncogene 24p3; p25; Siderocalin LCN2; MSFI |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln21-Gly198 |
Molecular Weight |
The protein has a predicted MW of 47.2 kDa. Due to glycosylation, the protein migrates to 48-52 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human NGAL, hFc Tag at 1 ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-NGAL Antibody, hFc Tag with the EC50 21.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in 50mM MES,150mM NaCl (pH 6.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in 50mM MES,150mM NaCl (pH 6.5). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human NGAL, hFc Tag at 1 ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-NGAL Antibody, hFc Tag with the EC50 21.4ng/ml determined by ELISA. |
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Human NGAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human NGAL is greater than 95% as determined by SEC-HPLC. |
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