Name: Human SCF Protein (SCF-HE001)
Brand: KactusBio
SKU: SCF-HE001-100UG
Price: 223.3 USD
Availability: InStock

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE
Activity: Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.55ug/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Glu26-Ala189

Catalog #SCF-HE001

Size

100UG

500UG

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Human SCF Protein (SCF-HE001) Summary

Product Description	Recombinant Human SCF Protein is expressed from E.coli without tag.It contains Glu26-Ala189.
Product Background	Kit-ligand (Kitl) also known as steel factor, stem cell factor and mast cell growth factor plays a crucial role in the development and maintenance of the melanocyte lineage in adult skin. Kitl exerts permanent survival, proliferation and migration functions in Kit receptor-expressing melanocytes.
Product Category	Recombinant Protein / Cell therapy / Cell culture / Cytokines

Human SCF Protein (SCF-HE001) Specifications/Details

Protein	SCF
Synonyms	MGF; c-Kit ligand; sKITLG; KITLG; FPH2; KL-1; Kitl; SF; SHEP7; KL
Accession	P21583-1
Species	Human
Biotinylated	no
Amino Acid Range	Glu26-Ala189
Molecular Weight	The protein has a predicted MW of 18.45 kDa same as Bis-Tris PAGE result.
Activity	Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.55ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE
Endotoxin	Less than 0.02 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human SCF at 5ug/ml (100ul/well) on the plate. Dose response curve for Human CD117, hFc Tag with the EC50 of 11.55ug/ml determined by ELISA (QC Test).
	Human SCF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured in a cell proliferation assay using Mo7e cells. The ED50-for this effect is 1-8 ng/mL (QC Test).