Name: Human Sonic Hedgehog (Shh) Protein (SHH-HE001)
Brand: KactusBio
SKU: SHH-HE001-100UG
Price: 297.0 USD
Availability: InStock

Human Sonic Hedgehog (Shh) Protein (SHH-HE001)

Human Sonic Hedgehog (Shh) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50-for this effect is 0.1-0.4 µg/mL.

The purity of Human Sonic Hedgehog (Shh) is greater than 95% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Amino Acid Range: Cys24-Gly197

Catalog #SHH-HE001

Size

100UG

500UG

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Product Description	Recombinant Human Sonic Hedgehog (Shh) Protein is expressed from E.coli without tag.It contains Cys24-Gly197.
Product Background	Sonic hedgehog (Shh) is a secreted protein with important roles in mammalian embryogenesis. During tooth development, Shh is primarily expressed in the dental epithelium, from initiation to the root formation stages.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	Sonic Hedgehog (Shh)
Synonyms	SHH; Sonic Hedgehog; HHG-1; Sonic hedgehog; HLP3; HPE3; SMMCI; TPT; TPTPS
Accession	Q15465
Species	Human
Biotinylated	no
Amino Acid Range	Cys24-Gly197
Molecular Weight	The protein has a predicted MW of 19.79 kDa kDa. The protein migrates to 23-25 kDa based on Bis-Tris PAGE result.
Product Tag	No Tag
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.05 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 300mM NaCl (pH 7.4). Normally 8% trehalose / 8% mannitol is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human Sonic Hedgehog (Shh) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50-for this effect is 0.1-0.4 µg/mL.
	The purity of Human Sonic Hedgehog (Shh) is greater than 95% as determined by SEC-HPLC.