Name: Human TNFSF15 Trimer Protein (FSF-HM415)
Brand: KactusBio
SKU: FSF-HM415-100UG
Price: 330.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-His-Flag
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, hFc Tag with the EC50 of 2.2ug/ml determined by ELISA. The affinity constant of 0.10 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Asp91-Leu251

Catalog #FSF-HM415

Size

100UG

500UG

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Product Description	Recombinant Human TNFSF15 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-terminus.It contains Asp91-Leu251 trimer design.
Product Background	TNF superfamily member 15 (TNFSF15), a cytokine largely produced by vascular endothelial cells and a specific inhibitor of the proliferation of these same cells, can inhibit VEGF-induced vascular permeability in vitro and in vivo, and that death receptor 3 (DR3), a cell surface receptor of TNFSF15, mediates TNFSF15-induced dephosphorylation of VEGFR2.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	TNFSF15 Trimer
Synonyms	TL1A; VEGI-251; TNFSF15; TL1; VEGI; VEGI192A
Accession	O95150-1
Species	Human
Biotinylated	no
Amino Acid Range	Asp91-Leu251
Molecular Weight	The protein has a predicted MW of 58.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, hFc Tag with the EC50 of 2.2ug/ml determined by ELISA. The affinity constant of 0.10 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Flag
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human DR3, hFc Tag captured on CM5 Chip via Protein A can bind Human TNFSF15 Trimer, His Tag with an affinity constant of 0.10 nM as determined in SPR assay (Biacore T200).
	Mouse DR3, His Tag immobilized on CM5 Chip can bind Human TNFSF15 (Trimer) , His Tag with an affinity constant of 3.06 nM as determined in SPR assay (Biacore T200).
	Immobilized Human TNFSF15 Trimer, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human DR3, hFc Tag with the EC50 of 2.2ug/ml determined by ELISA.
	Immobilized Human TNFSF15 Trimer, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-TNFSF15 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA.
	Immobilized Human TNFSF15 (Trimer), His Tag at 1ug/ml(100ul/well) on the plate. Dose response curve for Human DcR3, hFc Tag with the EC50 of 38.5ng/ml determined by ELISA.
	Human TNFSF15 Trimer on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human TNFSF15 Trimer is greater than 95% as determined by SEC-HPLC.