Human TSLP Protein (TSP-HM401)
Human TSLP Protein (TSP-HM401)
Human TSLP Protein (TSP-HM401)
Human TSLP Protein (TSP-HM401)
Human TSLP Protein (TSP-HM401)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TSLP, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA (QC Test). The affinity constant of 1.04 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Tyr29-Gln159
Catalog #TSP-HM401
Regular price $315.00
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Product Description
Recombinant Human TSLP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr29-Gln159.
Product Background
Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.
Product Category
Recombinant Protein
Target
TSLP
Synonyms
thymic stromal lymphopoietin; TSLP
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Tyr29-Gln159
Molecular Weight
The protein has a predicted MW of 17.8 kDa. Due to furin cleavage site, the protein migrates to 12 kDa&20-25 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human TSLP, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA (QC Test). The affinity constant of 1.04 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TSLP, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human TSLPR, hFc Tag with an affinity constant of 1.04 nM as determined in SPR assay (Biacore T200).
Immobilized Human TSLP, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA (QC Test).
Immobilized Human TSLP, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA.
Human TSLP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TSLP is greater than 95% as determined by SEC-HPLC.
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