Human TSLP Protein (TSP-HM401)
Human TSLP Protein (TSP-HM401)
Human TSLP Protein (TSP-HM401)
Human TSLP Protein (TSP-HM401)
Human TSLP Protein (TSP-HM401)
Human TSLP Protein (TSP-HM401)
Human TSLP Protein (TSP-HM401)
Human TSLP Protein (TSP-HM401)
Human TSLP Protein (TSP-HM401)
Human TSLP Protein (TSP-HM401)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human TSLP, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA (QC Test). The affinity constant of 1.04 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Tyr29-Gln159
Catalog #TSP-HM401
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Product Description
 Recombinant Human TSLP Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Tyr29-Gln159.
Product Background
 Thymic Stromal Lymphopoietin (TSLP) was originally identified as an activity from the conditioned medium of a mouse thymic stromal cell line that promoted the development of B cells. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c dendritic cells. Can induce allergic inflammation by directly activating mast cells.
Product Category
 Recombinant Protein
Target
 TSLP
Synonyms
 thymic stromal lymphopoietin; TSLP
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Tyr29-Gln159
Molecular Weight
 The protein has a predicted MW of 17.8 kDa. Due to furin cleavage site, the protein migrates to 12 kDa&20-25 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human TSLP, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA (QC Test). The affinity constant of 1.04 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-His-Avi
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human TSLP, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human TSLPR, hFc Tag with an affinity constant of 1.04 nM as determined in SPR assay (Biacore T200).
Immobilized Human TSLP, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human TSLPR, hFc Tag with the EC50 of 50.8ng/ml determined by ELISA (QC Test).
Immobilized Human TSLP, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-TSLP Antibody, hFc Tag with the EC50 of 13.0ng/ml determined by ELISA.
Human TSLP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human TSLP is greater than 95% as determined by SEC-HPLC.
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