Human IL-1F10/IL-38-Protein (ILF-HY138)

Express System: Yeast
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 35.38 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Met1-Trp152

Catalog: ILF-HY138

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Product Description	Recombinant Human IL-1F10/IL-38-Protein is expressed from Yeast with His tag at the C-terminus.It contains Met1-Trp152.
Product Background	Interleukin (IL)-38, a newly discovered IL-1 family cytokine, is expressed in several tissues and secreted by various cells. IL-38 has recently been reported to exert an anti-inflammatory function by binding to several receptors, including interleukin-36 receptor (IL-36R), interleukin-1 receptor accessory protein-like 1 (IL-1RAPL1), and interleukin-1 receptor 1 (IL-1R1) to block binding with other pro-inflammatory cytokines and inhibit subsequent signaling pathways; thereby regulating the differentiation and function of T cells, peripheral blood mononuclear cells, macrophages, and dendritic cells.
Product Category	Recombinant Protein

Target	IL-1F10/IL-38
Gene/Protein ID	AAK68048.1
Species	Human
Exact Sequence	Met1-Trp152
Molecular Weight	The protein has a predicted MW of 18.07 kDa. Due to glycosylation, the protein migrates to 20-23 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 35.38 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	Yeast
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-1 Rrp-2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1F10, His Tag with an affinity constant of 35.38 uM as determined in SPR assay (Biacore T200).
	The purity of Human IL-1F10 is greater than 95% as determined by SEC-HPLC.
	Human IL-1F10 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.