- Express System: Yeast
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
- Activity: The affinity constant of 35.38 uM as determined in SPR assay (Biacore T200). See testing image for detail.
- Amino Acid Range: Met1-Trp152
Product Description |
Recombinant Human IL-1F10/IL-38-Protein is expressed from Yeast with His tag at the C-terminus.It contains Met1-Trp152. |
Product Background |
Interleukin (IL)-38, a newly discovered IL-1 family cytokine, is expressed in several tissues and secreted by various cells. IL-38 has recently been reported to exert an anti-inflammatory function by binding to several receptors, including interleukin-36 receptor (IL-36R), interleukin-1 receptor accessory protein-like 1 (IL-1RAPL1), and interleukin-1 receptor 1 (IL-1R1) to block binding with other pro-inflammatory cytokines and inhibit subsequent signaling pathways; thereby regulating the differentiation and function of T cells, peripheral blood mononuclear cells, macrophages, and dendritic cells. |
Product Category |
Recombinant Protein |
Target |
IL-1F10/IL-38 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Met1-Trp152 |
Molecular Weight |
The protein has a predicted MW of 18.07 kDa. Due to glycosylation, the protein migrates to 20-23 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 35.38 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
Yeast |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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