• Express System: Yeast
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: The affinity constant of 35.38 uM as determined in SPR assay (Biacore T200).
  • Amino Acid Range: Met1-Trp152
Catalog #ILF-HY138
Price
Regular price $264.00
Regular price Sale price $264.00
/
Size
Avantor VWR Fisher Scientific

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Human IL-1F10/IL-38-Protein (ILF-HY138) Summary

Product Description

 Recombinant Human IL-1F10/IL-38-Protein is expressed from Yeast with His tag at the C-terminus.It contains Met1-Trp152.

Product Background

 Interleukin (IL)-38, a newly discovered IL-1 family cytokine, is expressed in several tissues and secreted by various cells. IL-38 has recently been reported to exert an anti-inflammatory function by binding to several receptors, including interleukin-36 receptor (IL-36R), interleukin-1 receptor accessory protein-like 1 (IL-1RAPL1), and interleukin-1 receptor 1 (IL-1R1) to block binding with other pro-inflammatory cytokines and inhibit subsequent signaling pathways; thereby regulating the differentiation and function of T cells, peripheral blood mononuclear cells, macrophages, and dendritic cells.

Product Category

 Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human IL-1F10/IL-38-Protein (ILF-HY138) Specifications/Details

Protein
 IL-1F10/IL-38
Synonyms
 FIL1 theta; IL-1HY2; IL-1 theta; IL38;
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Met1-Trp152
Molecular Weight
 The protein has a predicted MW of 18.07 kDa. Due to glycosylation, the protein migrates to 20-23 kDa based on Bis-Tris PAGE result.
Activity
 The affinity constant of 35.38 uM as determined in SPR assay (Biacore T200).
Product Tag
 C-His

Fluorophore

 Unconjugated
Expression System
 Yeast
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS, 1mM DTT (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Human IL-1 Rrp-2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1F10, His Tag with an affinity constant of 35.38 uM as determined in SPR assay (Biacore T200). Human IL-1 Rrp-2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1F10, His Tag with an affinity constant of 35.38 uM as determined in SPR assay (Biacore T200).
Human IL-1F10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-1F10 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-1F10 is greater than 95% as determined by SEC-HPLC. The purity of Human IL-1F10 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...
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