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Mouse B7-1/CD80 Protein (B71-MM180) Summary
Product Description |
Recombinant Mouse B7-1/CD80 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val38-Asn246. |
Product Background |
Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4. |
Product Category |
Recombinant Protein / Antibody discovery / Immune checkpoint proteins |
Mouse B7-1/CD80 Protein (B71-MM180) Specifications/Details
Protein |
B7-1/CD80 |
Synonyms |
CD80 molecule; B7; B71; B7-1; BB1; CD28LG1; CD80; B7.1; CD28LG1; LAB7 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Val38-Asn246 |
Molecular Weight |
The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Mouse B7-1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Mouse B7-1 is greater than 95% as determined by SEC-HPLC. |
Other Available Proteins:
Finding available proteins...
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