- Express System: E.coli
- Product Tag: C-His
- Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
- Activity: The affinity constant of 33.06 nM as determined in SPR assay (Biacore T200). See testing image for detail.
- Amino Acid Range: Ala1-Ser195
Product Description |
Recombinant Mouse BID Protein is expressed from E.coli with His tag at the C-Terminus.It contains Ala1-Ser195. |
Product Background |
Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
BID |
Synonyms |
p22 BID; BID; |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ala1-Ser195 |
Molecular Weight |
The protein has a predicted MW of 22.72 kDa same as Bis-Tris PAGE result. |
Activity |
The affinity constant of 33.06 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Expression System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
More from Antibody Drug Discovery Proteins
Recently viewed