Mouse BID Protein (BID-ME101)
Mouse BID Protein (BID-ME101)
  • Express System: E.coli
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: The affinity constant of 33.06 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ala1-Ser195
Catalog #BID-ME101
Regular price $379.50
/
Size

Order now, ships by:

Product Description

Recombinant Mouse BID Protein is expressed from E.coli with His tag at the C-Terminus.It contains Ala1-Ser195.

Product Background

Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals.

Product Category

Recombinant Protein / Antibody discovery

Protein

BID

Synonyms

p22 BID; BID;

Accession

Species

Mouse

Biotinylated

no

Amino Acid Range

Ala1-Ser195

Molecular Weight

The protein has a predicted MW of 22.72 kDa same as Bis-Tris PAGE result.

Activity

The affinity constant of 33.06 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Expression System

E.coli

Purity

> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse BID, His Tag immobilized on CM5 Chip can bind Human BCL2L1, His Tag with an affinity constant of 33.06 nM as determined in SPR assay (Biacore T200). Mouse BID, His Tag immobilized on CM5 Chip can bind Human BCL2L1, His Tag with an affinity constant of 33.06 nM as determined in SPR assay (Biacore T200).
Mouse BID on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse BID on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse BID is greater than 90% as determined by SEC-HPLC. The purity of Mouse BID is greater than 90% as determined by SEC-HPLC.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed