Product Description |
Recombinant Mouse BID Protein is expressed from E.coli with His tag at the C-Terminus.It contains Ala1-Ser195. |
Product Background |
Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. |
Product Category |
Recombinant Protein |
Target |
BID |
Synonyms |
p22 BID; BID; |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ala1-Ser195 |
Molecular Weight |
The protein has a predicted MW of 22.72 kDa same as Bis-Tris PAGE result. |
Activity |
The affinity constant of 33.06 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
E.coli |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Mouse BID, His Tag immobilized on CM5 Chip can bind Human BCL2L1, His Tag with an affinity constant of 33.06 nM as determined in SPR assay (Biacore T200). |
|
|
Mouse BID on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Mouse BID is greater than 90% as determined by SEC-HPLC. |
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