Mouse BID Protein (BID-ME101)

Express System: E.coli
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Activity: The affinity constant of 33.06 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ala1-Ser195

Catalog: BID-ME101

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Product Description	Recombinant Mouse BID Protein is expressed from E.coli with His tag at the C-Terminus.It contains Ala1-Ser195.
Product Background	Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals.
Product Category	Recombinant Protein

Target	BID
Gene/Protein ID	EDK99650.1
Species	Mouse
Exact Sequence	Ala1-Ser195
Molecular Weight	The protein has a predicted MW of 22.72 kDa same as Tris-Bis PAGE result.
Activity	The affinity constant of 33.06 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Mouse BID, His Tag immobilized on CM5 Chip can bind Human BCL2L1/Bcl-XL Protein, His Tag with an affinity constant of 33.06 nM as determined in SPR assay (Biacore T200).
	The purity of Mouse BID is greater than 90% as determined by SEC-HPLC.
	Mouse BID on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.