Mouse BID Protein (BID-ME101)

Mouse BID, His Tag immobilized on CM5 Chip can bind Human BCL2L1, His Tag with an affinity constant of 33.06 nM as determined in SPR assay (Biacore T200).

Mouse BID on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Mouse BID is greater than 90% as determined by SEC-HPLC.

Express System: E.coli
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: The affinity constant of 33.06 nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Ala1-Ser195

Catalog #BID-ME101

Price

$379⁵⁰

Size

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Mouse BID Protein (BID-ME101) Summary

Product Description

Recombinant Mouse BID Protein is expressed from E.coli with His tag at the C-Terminus.It contains Ala1-Ser195.

Product Background

Bid, BH3-interacting domain death agonist, was initially cloned based in its ability to interact with both Bcl-2 and Bax. Bid contains only the BH3 domain, which is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. Bid is susceptible to proteolytic cleavage by caspases, calpains, Granzyme B and cathepsins. Bid is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals.

Mouse BID Protein (BID-ME101) Specifications/Details

Protein	BID
Synonyms	p22 BID; BID;;p22BID
Accession	EDK99650.1
Species	Mouse
Biotinylated	no
Amino Acid Range	Ala1-Ser195
Molecular Weight	The protein has a predicted MW of 22.72 kDa same as Bis-Tris PAGE result.
Activity	The affinity constant of 33.06 nM as determined in SPR assay (Biacore T200).
Product Tag	C-His
Fluorophore	Unconjugated
Expression System	E.coli
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Mouse BID, His Tag immobilized on CM5 Chip can bind Human BCL2L1, His Tag with an affinity constant of 33.06 nM as determined in SPR assay (Biacore T200).
	Mouse BID on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Mouse BID is greater than 90% as determined by SEC-HPLC.