Product Description |
Recombinant Mouse IL-2 Protein is expressed from HEK293 without tag.It contains Ala21-Gln169. |
Product Background |
Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells. |
Product Category |
Recombinant Protein / Cell therapy / Cell culture / Cytokines |
Protein |
IL-2 |
Synonyms |
IL2; T-cell growth factor; TCGF; Aldesleukin |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Ala21-Gln169 |
Molecular Weight |
The protein has a predicted MW of 17.23 kDa. Due to glycosylation, the protein migrates to 23-28 kDa based on Bis-Tris PAGE result. |
Product Tag |
No Tag |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
|
|
Mouse IL-2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 0.4 - 1 ng/mL (QC Test). |
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The purity of Mouse IL-2 is greater than 95% as determined by SEC-HPLC. |
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