Product Description |
Recombinant Mouse Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Phe31-Val988(ITGAV) acidic tail & Glu26-Asp717(ITGB3) basic tail. |
Product Background |
ITGAV&ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling. ITGAV&ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling. ITGAV&ITGB3 binds to FGF2 and this binding is essential for FGF2 signaling. ITGAV&ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling. ITGAV&ITGB3 binds to IGF2 and this binding is essential for IGF2 signaling. |
Product Category |
Recombinant Protein |
Target |
Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer |
Synonyms |
αVβ3; Integrin alpha V beta 3; beta3; alphaV; ITGAV&ITGB3; Integrin alpha V β 3; β3 |
Accession |
|
Species |
Mouse |
Biotinylated |
no |
Amino Acid Range |
Phe31-Val988(ITGAV) acidic tail & Glu26-Asp717(ITGB3) basic tail |
Molecular Weight |
The protein has a predicted MW of 131.48 kDa (ITGAV) & 101.58 kDa (ITGB3). Due to enzyme lysis and glycosylation, the protein migrates to 110-135 kDa and 50-60 kDa based on Bis-Tris PAGE result. |
Activity |
The affinity constant of 3.37 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Mouse ITGAV&ITGB3, hFc Tag captured on CM5 Chip via Protein A can bind Human Vitronectin, His Tag with an affinity constant of 3.37 nM as determined in SPR assay (Biacore T200). |
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Mouse ITGAV&ITGB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Mouse ITGAV&ITGB3 is greater than 95% as determined by SEC-HPLC. |
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