SDS-PAGE gel image showing protein molecular weight marker (MK) and recombinant protein (R) with visible bands around 50 kDa and 40 kDa.
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Amino Acid Range: Ala25-Asp441
Catalog #MEP-MM101
Regular price $297.00
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Product Description

Recombinant Mouse MEPE Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala25-Asp441.

Product Background

Matrix extracellular phosphoglycoprotein (MEPE) is expressed in bone and teeth where it has multiple functions. The C-terminus of MEPE contains a mineral-binding, acidic serine- and aspartate-rich motif (ASARM) that is also present in other noncollagenous proteins of mineralized tissues.MEPE-derived ASARM peptides function in phosphate homeostasis and direct inhibition of bone mineralization in a phosphorylation-dependent manner.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
MEPE
Synonyms
OF45; MEPE; Osteoregulin
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Ala25-Asp441
Molecular Weight
The protein has a predicted MW of 45.3 kDa. The protein is cleaved into 2 chains and due to glycosylation, it migrates to 45-60 kDa based on Bis-Tris PAGE result.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Mouse MEPE on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse MEPE on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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