ELISA binding curve of mouse OX-40 His tag protein showing mean absorbance versus log concentration
Non-biotinylated Mouse OX40/TNFRSF4/CD134 Protein (OX4-MM440)
Non-biotinylated Mouse OX40/TNFRSF4/CD134 Protein (OX4-MM440)
Non-biotinylated Mouse OX40/TNFRSF4/CD134 Protein (OX4-MM440)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Mouse OX40 Ligand, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse OX40, His Tag with the EC50 of 1.06ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Val20-Pro211
Catalog #OX4-MM440
Regular price $231.00
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Product Description

Recombinant Mouse OX40/TNFRSF4/CD134 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Val20-Pro211.

Product Background

Tumor necrosis factor receptor superfamily, member 4 (TNFRSF4), also known as CD134 and OX40 receptor. OX40 is a secondary co-stimulatory immune checkpoint molecule, expressed after 24 to 72 hours following activation; its ligand, OX40L, is also not expressed on resting antigen presenting cells, but is following their activation.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins
Protein
OX40/TNFRSF4/CD134
Synonyms
CD134; OX40; OX40L receptor; TNFRSF4; ACT-135; Ly-70; OX40 homologue;-TXGP1L; IMD16
Accession
Species
Mouse
Biotinylated
no
Amino Acid Range
Val20-Pro211
Molecular Weight
The protein has a predicted MW of 24.2 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Mouse OX40 Ligand, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse OX40, His Tag with the EC50 of 1.06ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Mouse OX40 Ligand, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse OX40, His Tag with the EC50 of 1.06ug/ml determined by ELISA. Immobilized Mouse OX40 Ligand, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Mouse OX40, His Tag with the EC50 of 1.06ug/ml determined by ELISA.
Mouse OX40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Mouse OX40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Mouse OX40 is greater than 90% as determined by SEC-HPLC. The purity of Mouse OX40 is greater than 90% as determined by SEC-HPLC.
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