SARS-COV-2 Spike S1 Protein (COV-VM5S1)
Catalog: COV-VM5S1
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| Product Description | Recombinant SARS-COV-2 Spike S1 Protein is expressed from Expi293 with hFc tag and Avi tag at the C-terminal.It contains Gln14-Arg683. |
| Product Category | Recombinant Protein |
| Target | Spike S1 |
| Synonyms | S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1 |
| Gene/Protein ID | QHD43416.1 |
| Species | SARS-COV-2 |
| Exact Sequence | Gln14-Arg683 |
| Molecular Weight | The protein has a predicted MW of 102.6 kDa. Due to glycosylation, the protein migrates to 130-140 kDa based on Tris-Bis PAGE result. |
| Activity | The affinity constant of 3.80 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
| Product Tag | C-hFc-Avi |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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SARS-COV-2 Spike S1, hFc Tag captured on CM5 Chip via Protein A can bind Human ACE2, His Tag with an affinity constant of 3.80 nM as determined in SPR assay (Biacore T200). |
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The purity of SARS-COV-2 Spike S1 is greater than 95% as determined by SEC-HPLC. |
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SARS-COV-2 Spike S1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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