SARS-COV-2 Spike S1 Protein (COV-VM5S1)

Express System: HEK293
Product Tag: C-hFc-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 92.3ng/ml determined by ELISA. The affinity constant of 3.80 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gln14-Arg683

Catalog: COV-VM5S1

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Product Description	Recombinant SARS-COV-2 Spike S1 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Gln14-Arg683.
Product Background	The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Product Category	Recombinant Protein

Target	Spike S1
Synonyms	S1 protein; Spike protein S1; Spike,S1 protein; S glycoprotein Subunit1
Gene/Protein ID	QHD43416.1
Species	SARS-COV-2
Exact Sequence	Gln14-Arg683
Molecular Weight	The protein has a predicted MW of 102.6 kDa. Due to glycosylation, the protein migrates to 130-140 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 92.3ng/ml determined by ELISA. The affinity constant of 3.80 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	SARS-COV-2 Spike S1, hFc Tag captured on CM5 Chip via Protein A can bind Human ACE2, His Tag with an affinity constant of 3.80 nM as determined in SPR assay (Biacore T200).
	Immobilized Human ACE2, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for SARS-COV-2 Spike S1, hFc Tag with the EC50 of 92.3ng/ml determined by ELISA.
	The purity of SARS-COV-2 Spike S1 is greater than 95% as determined by SEC-HPLC.
	SARS-COV-2 Spike S1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.