Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein (GFL-CM401B)
Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein (GFL-CM401B)
  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Cynomolgus GDF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln20-Glu351
Catalog #GFL-CM401B
Regular price $600.00
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Product Description

Recombinant Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gln20-Glu351.

Product Background

GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.

Product Category

Recombinant Protein / Antibody discovery

Protein

GFRAL/GFR alpha-like

Synonyms

GFR alpha-like; GFRAL; GRAL; C6orf144

Accession

Species

Cynomolgus

Biotinylated

yes

Amino Acid Range

Gln20-Glu351

Molecular Weight

The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Cynomolgus GDF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His-Avi

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1EU per ug by the LAL method.
Form
Liquid
Shipping
Shipped with dry ice.
Formulation
Supplied as 0.22um filtered solution in 20mM PB, 400mM NaCl (pH 6.0).
Stability And Storage
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Cynomolgus GDF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA. Immobilized Cynomolgus GDF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA.
Biotinylated Cynomolgus GFRAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Cynomolgus GFRAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Biotinylated Cynomolgus GFRAL is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Cynomolgus GFRAL is greater than 95% as determined by SEC-HPLC.

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