Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein (GFL-CM401B)

  • Express System: HEK293
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Biotinylated: yes
  • Activity: Immobilized Cynomolgus GDF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln20-Glu351

Catalog: GFL-CM401B

$600.00
Size:

Order now get it on

Bulk Quote | Test Sample
Product Description Recombinant Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gln20-Glu351.

Product Background

 GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.

Product Category

 Recombinant Protein

Target

 GFRAL/GFR alpha-like

Gene/Protein ID

 XP_015304775.2

Species

 Cynomolgus

Exact Sequence

 Gln20-Glu351
Molecular Weight The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Cynomolgus GDF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

 N-His-Avi

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Liquid
Shipping Shipped with dry ice.
Formulation Supplied as 0.22um filtered solution in 20mM PB, 400mM NaCl (pH 6.0).
Stability And Storage Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Cynomolgus GDF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA.
The purity of Biotinylated Cynomolgus GFRAL is greater than 95% as determined by SEC-HPLC.
Biotinylated Cynomolgus GFRAL on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in

View more associated products