Name: Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein (GFL-CM401B)
Brand: KactusBio
SKU: GFL-CM401B-100UG
Price: 600.0 USD
Availability: InStock

Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein (GFL-CM401B)

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Cynomolgus GDF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Gln20-Glu351

Catalog #GFL-CM401B

Size

100UG

500UG

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Product Description	Recombinant Biotinylated Cynomolgus GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gln20-Glu351.
Product Background	GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
Product Category	Recombinant Protein

Target	GFRAL/GFR alpha-like
Accession	XP_015304775.2
Species	Cynomolgus
Biotinylated	yes
Amino Acid Range	Gln20-Glu351
Molecular Weight	The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Cynomolgus GDF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Avi
Express System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22um filtered solution in 20mM PB, 400mM NaCl (pH 6.0).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Cynomolgus GDF15, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Cynomolgus GFRAL, His Tag with the EC50 of 101.8ng/ml determined by ELISA.
	Biotinylated Cynomolgus GFRAL on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Biotinylated Cynomolgus GFRAL is greater than 95% as determined by SEC-HPLC.

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