Biotinylated Human ALK-7/Activin RIC Protein (ALK-HM407B)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Biotinylated Human ALK-7, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ALK7 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Glu21-Glu113
Catalog #ALK-HM407B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human ALK-7/Activin RIC Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Glu21-Glu113.

Product Background

Arterial stiffness is an important feature of diabetic macrovascular complications. Activin receptor-like kinase 7 (ALK7), a member of type I transforming growth factor-β (TGF-β) receptors, is correlated with pathogenic risks of type 2 diabetes mellitus and cardiovascular diseases and may be involved in cardiovascular remodeling.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
ALK-7/Activin RIC
Synonyms
ACTR-IC; ACVR1C; ALK7; Activin R1C;
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Glu21-Glu113
Molecular Weight
The protein has a predicted MW of 13.71 kDa. Due to glycosylation, the protein migrates to 20-40 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Biotinylated Human ALK-7, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ALK7 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 20mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Biotinylated Human ALK-7, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ALK7 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test). Immobilized Biotinylated Human ALK-7, His-Avi Tag at 0.5ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-ALK7 Antibody, hFc Tag with the EC50 of 3.8ng/ml determined by ELISA (QC Test).
Biotinylated Human ALK-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human ALK-7 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Anti-ALK-7 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Biotinylated Human ALK-7, His Tag with an affinity constant of 1.84 nM as determined in BLI assay (Gator® Prime). Loaded Anti-ALK-7 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Biotinylated Human ALK-7, His Tag with an affinity constant of 1.84 nM as determined in BLI assay (Gator® Prime).
The purity of Biotinylated Human ALK-7 is greater than 95% as determined by SEC-HPLC. The purity of Biotinylated Human ALK-7 is greater than 95% as determined by SEC-HPLC.
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