SDS-PAGE gel showing protein ladder and one distinct purified protein band, indicating molecular weight and purity.
Biotinylated Human CXCL4 Protein (CXC-HM4L4B)
Biotinylated Human CXCL4 Protein (CXC-HM4L4B)
  • Express System: E.coli
  • Product Tag: N-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CCL5, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CXCL4, His Tag with the EC50 of 1.1ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Glu32-Ser101
Catalog #CXC-HM4L4B
Regular price $577.50
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Product Description

Recombinant Biotinylated Human CXCL4 Protein is expressed from E.coli with His tag and Avi tag at the N-Terminus.It contains Glu32-Ser101.

Product Background

Chemokines regulate leukocyte migration during physiological and pathological conditions. It is currently accepted that these chemotactic cytokines are also important in the development and progression of cancer. CXCL4 and its non-allelic variant CXCL4L1 are two platelet-associated chemokines that have been attributed anti-tumoral activity as a result of their angiostatic potential and the chemotactic activity for anti-tumoral leukocytes.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others
Protein
CXCL4
Synonyms
PF-4; Oncostatin-A; CXCL4; SCYB4; Iroplact; MGC138298
Accession
Species
Human
Biotinylated
yes
Amino Acid Range
Glu32-Ser101
Molecular Weight
The protein has a predicted MW of 10.68 kDa. The protein migrates to 13 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CCL5, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CXCL4, His Tag with the EC50 of 1.1ug/ml determined by ELISA. See testing image for detail.

Product Tag

N-His-Avi
Expression System
E.coli
Purity
> 95% as determined by Bis-Tris PAGE
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 4mM HCl (pH 3.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in 4mM HCL (pH 3.0). It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human CCL5, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CXCL4, His Tag with the EC50 of 1.1ug/ml determined by ELISA. Immobilized Human CCL5, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CXCL4, His Tag with the EC50 of 1.1ug/ml determined by ELISA.
Biotinylated Human CXCL4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Biotinylated Human CXCL4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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